Genetic elements are key components of metabolic engineering and synthetic biological
applications, allowing the development of organisms as biosensors and for manufacturing …

Synonymous mutation of the N-terminal coding sequence (NCS) has been used to regulate
gene expression. We here developed a statistical model to predict the effect of the NCSs on …

Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

Libraries of well-characterised components regulating gene expression levels are essential
to many synthetic biology applications. While widely available for the Gram-negative model …

We introduce a manually constructed and curated regulatory network model that describes
the current state of knowledge of transcriptional regulation of Bacillus subtilis. The model …

Promoters are among the most-important and most-basic tools for the control of metabolic
pathways. However, previous research mainly focused on the screening and …

Bacillus subtilis is the leading model Gram-positive bacterium, and a widely used chassis for
industrial protein production. However, B. subtilis research is limited by a lack of inducible …

Intense synthesis of proteins and chemicals in engineered microbes impose metabolic
burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the …

Bacillus subtilis is a generally recognized as safe microorganism that is widely used for
protein expression and chemical production, but has a limited number of genetic regulatory …

By combining advanced omics technology and computational modeling, systems biologists
have identified and inferred thousands of regulatory events and system-wide interactions of …