A non-α-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus

M Imamura, T Uemori, I Kato, Y Ishino - … and Pharmaceutical Bulletin, 1995 - jstage.jst.go.jp
抄録 We identified a DNA polymerase with properties that differed from those of α-like DNA
polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA …

The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases

T Uemori, Y Ishino, H Doi, I Kato - Journal of bacteriology, 1995 - Am Soc Microbiol
We cloned two genes encoding DNA polymerases from the hyperthermophilic archaeon
Pyrodictium occultum. The deduced primary structures of the two gene products have …

Comprehensive Search for DNA Polymerase in the Hyperthermophilic Archaeon, Pyrococcus furiosus

S Ishino, Y Ishino - Nucleosides, Nucleotides and Nucleic Acids, 2006 - Taylor & Francis
DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all
of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing …

Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi

Y Gueguen, J Rolland, O Lecompte… - European Journal of …, 2001 - Wiley Online Library
The complete genome sequence of the hyperthermophilic archaeon Pyrococcus abyssi
revealed the presence of a family B DNA polymerase (Pol I) and a family D DNA polymerase …

Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus

T Uemori, Y Ishino, H Toh, K Asada… - Nucleic acids …, 1993 - academic.oup.com
We cloned the gene encoding the thermostable DNA polymerase from the archaeon
Pyrococcus furiosus. The DNA fragment of 2785 base pair (bp) containing the structural …

A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization

T Uemori, Y Sato, I Kato, H Doi, Y Ishino - Genes to Cells, 1997 - Wiley Online Library
Background: In many respects Archaea are much more like eukaryotes than prokaryotes
with respect to the conservation of many of the components involved in transcription …

The DNA polymerase gene from the hyperthermophilic marine archaebacterium, Pyrococcus furiosus, shows sequence homology with alpha-like DNA polymerases.

EJ Mathur, MW Adams, WN Callen… - Nucleic acids …, 1991 - ncbi.nlm.nih.gov
Recent studies in the evolutionary relationships of life on this planet have placed the root of
the universal tree between eubacteria (or Bacteria) on the one hand and archaebacteria (or …

Cloning and Expression inEscherichia Coliof the Recombinant His-Tagged DNA Polymerases fromPyrococcus furiosusandPyrococcus Woesei

S Dąbrowski, J Kur - Protein expression and purification, 1998 - Elsevier
Complete PCR-derived DNA fragments containing the structural genes for DNA
polymerases of the archaeonsPyrococcus furiosusandPyrococcus woeseiwere cloned into …

Purification and characterization of a DNA polymerase from the archaebacterium Thermoplasma acidophilum

A Hamal, P Forterre, C Elie - European journal of biochemistry, 1990 - Wiley Online Library
A thermophilic DNA polymerase has been purified to near homogeneity from the
archaebacterium Thermoplasma acidophilum. Analysis of the purified enzyme by sodium …

Two Family B DNA Polymerases from Aeropyrum pernix, an Aerobic Hyperthermophilic Crenarchaeote

IKO Cann, S Ishino, N Nomura, Y Sako… - Journal of …, 1999 - Am Soc Microbiol
ABSTRACT DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a
hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and …