[HTML][HTML] The Archaeal DNA primase: biochemical characterization of the p41-p46 complex Frompyrococcus furiosus

L Liu, K Komori, S Ishino, AA Bocquier… - Journal of Biological …, 2001 - ASBMB
We characterized the primase complex of the hyperthermophilic archaeon, Pyrococcus
furiosus. The two proteins, Pfup41 and Pfup46, have similar sequences to the p48 and p58 …

The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair?

M Le Breton, G Henneke, C Norais, D Flament… - Journal of Molecular …, 2007 - Elsevier
We report on the characterization of the DNA primase complex of the hyperthermophilic
archaeon Pyrococcus abyssi (Pab). The Pab DNA primase complex is composed of the …

[HTML][HTML] Archaeal primase: bridging the gap between RNA and DNA polymerases

AA Bocquier, L Liu, IKO Cann, K Komori, D Kohda… - Current Biology, 2001 - cell.com
In the evolution of life, DNA replication is a fundamental process, by which species transfer
their genetic information to their offspring. DNA polymerases, including bacterial and …

Crystal structure of the Pyrococcus horikoshii DNA primase‐UTP complex: implications for the mechanism of primer synthesis

N Ito, O Nureki, M Shirouzu, S Yokoyama… - Genes to …, 2003 - Wiley Online Library
Background: In chromosomal DNA replication, DNA primase initiates the synthesis of a
dinucleotide on a single‐stranded template DNA, and elongates it to form a primer RNA for …

[HTML][HTML] Characterization of DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis

WC Galal, M Pan, Z Kelman, J Hurwitz - Journal of Biological Chemistry, 2012 - ASBMB
In most organisms, DNA replication is initiated by DNA primases, which synthesize primers
that are elongated by DNA polymerases. In this study, we describe the isolation and …

The DNA primase of Sulfolobus solfataricus is activated by substrates containing a thymine-rich bubble and has a 3′-terminal nucleotidyl-transferase activity

M De Falco, A Fusco, M De Felice, M Rossi… - Nucleic acids …, 2004 - academic.oup.com
DNA primases are responsible for the synthesis of the short RNA primers that are used by
the replicative DNA polymerases to initiate DNA synthesis on the leading-and lagging …

Distinct Domain Functions Regulating de Novo DNA Synthesis of Thermostable DNA Primase from Hyperthermophile Pyrococcus horikoshii

E Matsui, M Nishio, H Yokoyama, K Harata… - Biochemistry, 2003 - ACS Publications
DNA primases are essential components of the DNA replication apparatus in every
organism. Reported here are the biochemical characteristics of a thermostable DNA primase …

Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii

G Desogus, S Onesti, P Brick, M Rossi… - Nucleic acids …, 1999 - academic.oup.com
We report the identification and characterisation of a DNA primase from the thermophilic
methanogenic archaeon Methanococcus jannaschii (Mjpri). The analysis of the complete …

The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3′-terminal nucleotidyl …

S Lao-Sirieix, SD Bell - Journal of molecular biology, 2004 - Elsevier
A eukaryotic-type primase was identified in the crenarchaeon Sulfolobus solfataricus. The
two-subunit DNA-dependent primase, termed PriSL, was purified following co-expression of …

Properties of an unusual DNA primase from an archaeal plasmid

K Beck, G Lipps - Nucleic acids research, 2007 - academic.oup.com
Primases are specialized DNA-dependent RNA polymerases that synthesize a short
oligoribonucleotide complementary to single-stranded template DNA. In the context of …