Development of Additional Selectable Markers for the Halophilic Archaeon Haloferax volcanii Based on the leuB and trpA Genes

T Allers, HP Ngo, M Mevarech… - Applied and …, 2004 - Am Soc Microbiol
Since most archaea are extremophilic and difficult to cultivate, our current knowledge of their
biology is confined largely to comparative genomics and biochemistry. Haloferax volcanii …

Development of a Gene Knockout System for the Halophilic Archaeon Haloferax volcanii by Use of the pyrE Gene

G Bitan-Banin, R Ortenberg, M Mevarech - Journal of bacteriology, 2003 - Am Soc Microbiol
So far, the extremely halophilic archaeon Haloferax volcanii has the best genetic tools
among the archaea. However, the lack of an efficient gene knockout system for this …

Improved Strains and Plasmid Vectors for Conditional Overexpression of His-Tagged Proteins in Haloferax volcanii

T Allers, S Barak, S Liddell, K Wardell… - Applied and …, 2010 - Am Soc Microbiol
Research into archaea will not achieve its full potential until systems are in place to carry out
genetics and biochemistry in the same species. Haloferax volcanii is widely regarded as the …

Characterization of a tightly controlled promoter of the halophilic archaeon Haloferax volcanii and its use in the analysis of the essential cct1 gene

A Large, C Stamme, C Lange, Z Duan… - Molecular …, 2007 - Wiley Online Library
A system where archaeal gene expression could be controlled by simple manipulation of
growth conditions would enable the construction of conditional lethal mutants in essential …

[PDF][PDF] Genetic transfer in Halobacterium volcanii

M Mevarech, R Werczberger - Journal of Bacteriology, 1985 - Am Soc Microbiol
Genetic Transfer in Halobacterium volcanii Page 1 JOURNAL OF BACTERIOLOGY, Apr. 1985,
p. 461-462 Vol. 162, No. 1 0021-9193/85/040461-02$02.00/0 Copyright X 1985, American …

Construction and usage of a onefold‐coverage shotgun DNA microarray to characterize the metabolism of the archaeon Haloferax volcanii

A Zaigler, SC Schuster, J Soppa - Molecular microbiology, 2003 - Wiley Online Library
Haloferax volcanii is a moderately halophilic archaeon that can grow aerobically and
anaerobically with a variety of substrates. We undertook a novel approach for the …

The Complete Genome Sequence of Haloferax volcanii DS2, a Model Archaeon

AL Hartman, C Norais, JH Badger, S Delmas… - PloS one, 2010 - journals.plos.org
Background Haloferax volcanii is an easily culturable moderate halophile that grows on
simple defined media, is readily transformable, and has a relatively stable genome. This, in …

Development of pyrF-based gene knockout systems for genome-wide manipulation of the archaea Haloferax mediterranei and Haloarcula hispanica

H Liu, J Han, X Liu, J Zhou, H Xiang - Journal of Genetics and Genomics, 2011 - Elsevier
The haloarchaea Haloferax mediterranei and Haloarcula hispanica are both
polyhydroxyalkanoate producers in the domain Archaea, and they are becoming …

From genomes to function: haloarchaea as model organisms

J Soppa - Microbiology, 2006 - microbiologyresearch.org
Haloarchaea are adapted to high-salt environments and accumulate equally high salt
concentrations in the cytoplasm. The genomes of representatives of six haloarchaeal genera …

Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea

NS Baliga, R Bonneau, MT Facciotti, M Pan… - Genome …, 2004 - genome.cshlp.org
We report the complete sequence of the 4,274,642-bp genome of Haloarcula marismortui, a
halophilic archaeal isolate from the Dead Sea. The genome is organized into nine circular …