[HTML][HTML] CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter
DNA hypermethylation at the promoter of tumour-suppressor genes is tightly correlated with
their transcriptional repression and recognized as the hallmark of majority of cancers …
their transcriptional repression and recognized as the hallmark of majority of cancers …
Repurposing the CRISPR-Cas9 system for targeted DNA methylation
Epigenetic studies relied so far on correlations between epigenetic marks and gene
expression pattern. Technologies developed for epigenome editing now enable direct study …
expression pattern. Technologies developed for epigenome editing now enable direct study …
Gene-specific targeting of DNA methylation in the mammalian genome
A Urbano, J Smith, RJ Weeks, A Chatterjee - Cancers, 2019 - mdpi.com
DNA methylation is the most widely-studied epigenetic modification, playing a critical role in
the regulation of gene expression. Dysregulation of DNA methylation is implicated in the …
the regulation of gene expression. Dysregulation of DNA methylation is implicated in the …
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
DM Sapozhnikov, M Szyf - Nature Communications, 2021 - nature.com
Despite four decades of research to support the association between DNA methylation and
gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that …
gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that …
A CRISPR-based approach for targeted DNA demethylation
X Xu, Y Tao, X Gao, L Zhang, X Li, W Zou, K Ruan… - Cell discovery, 2016 - nature.com
In mammalian cells, DNA methylation critically regulates gene expression and thus has
pivotal roles in myriad of physiological and pathological processes. Here we report a novel …
pivotal roles in myriad of physiological and pathological processes. Here we report a novel …
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases
Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …
Efficient targeted DNA methylation with chimeric dCas9–Dnmt3a–Dnmt3L methyltransferase
DNA methylation plays a critical role in the regulation and maintenance of cell-type specific
transcriptional programs. Targeted epigenome editing is an emerging technology to …
transcriptional programs. Targeted epigenome editing is an emerging technology to …
Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system
Establishing causal relationship between epigenetic marks and gene transcription requires
molecular tools, which can precisely modify specific genomic regions. Here, we present a …
molecular tools, which can precisely modify specific genomic regions. Here, we present a …
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs
Detection of DNA methylation in the genome has been possible for decades; however, the
ability to deliberately and specifically manipulate local DNA methylation states in the …
ability to deliberately and specifically manipulate local DNA methylation states in the …
Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation
Advances in sequencing technology allow researchers to map genome-wide changes in
DNA methylation in development and disease. However, there is a lack of experimental …
DNA methylation in development and disease. However, there is a lack of experimental …