In vitro generation and type-specific neutralization of a human papillomavirus type 16 virion pseudotype

RB Roden, HL Greenstone, R Kirnbauer… - Journal of …, 1996 - Am Soc Microbiol
We report a system for generating infectious papillomaviruses in vitro that facilitates the
analysis of papillomavirus assembly, infectivity, and serologic relatedness. Cultured hamster …

Characterization of a human papillomavirus type 16 variant-dependent neutralizing epitope

RB Roden, A Armstrong, P Haderer… - Journal of …, 1997 - Am Soc Microbiol
We have determined that three type-specific and conformationally dependent monoclonal
antibodies, H16. E70, H16. U4, and H16. V5, neutralize pseudotype human papillomavirus …

Common neutralization epitope in minor capsid protein L2 of human papillomavirus types 16 and 6

K Kawana, H Yoshikawa, Y Taketani… - Journal of …, 1999 - Am Soc Microbiol
Studies of virus neutralization by antibody are a prerequisite for development of a
prophylactic vaccine strategy against human papillomaviruses (HPVs). Using HPV16 and-6 …

In vitro infection and type-restricted antibody-mediated neutralization of authentic human papillomavirus type 16

WI White, SD Wilson, W Bonnez, RC Rose… - Journal of …, 1998 - Am Soc Microbiol
ABSTRACT Human papillomavirus type 16 (HPV-16) is strongly associated with the
development of cervical cancer. Studies of model systems with animal papillomaviruses …

L1 interaction domains of papillomavirus l2 necessary for viral genome encapsidation

MM Okun, PM Day, HL Greenstone, FP Booy… - Journal of …, 2001 - Am Soc Microbiol
ABSTRACT BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome
and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid …

Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles

RC Rose, W Bonnez, RC Reichman… - Journal of virology, 1993 - Am Soc Microbiol
The L1 coat protein of human papillomavirus type 11 (HPV-11) was expressed in Sf-9 insect
cells with the recombinant baculovirus vector Ac11L1. Viruslike particles (VLPs) were …

Cell surface-binding motifs of L2 that facilitate papillomavirus infection

R Yang, PM Day, WH Yutzy IV, KY Lin, CF Hung… - Journal of …, 2003 - Am Soc Microbiol
ABSTRACT Human papillomavirus type 16 (HPV16) is the primary etiologic agent of cervical
carcinoma, whereas bovine papillomavirus type 1 (BPV1) causes benign fibropapillomas …

Efficient intracellular assembly of papillomaviral vectors

CB Buck, DV Pastrana, DR Lowy, JT Schiller - Journal of virology, 2004 - Am Soc Microbiol
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble
to form virus-like particles, currently available methods for production of L1/L2 particles …

Human papillomavirus types 6 and 11 have antigenically distinct strongly immunogenic conformationally dependent neutralizing epitopes

ND Christensen, R Kirnbauer, JT Schiller, SJ Ghim… - Virology, 1994 - Elsevier
Antibodies reactive to HPV types 6 and 11 were tested in ELISA and HPV-11 neutralization
assays to determine whether these closely related types shared cross-reactive neutralizing …

In vitro construction of pseudovirions of human papillomavirus type 16: incorporation of plasmid DNA into reassembled L1/L2 capsids

K Kawana, H Yoshikawa, Y Taketani… - Journal of …, 1998 - Am Soc Microbiol
Lack of permissive and productive cell cultures for the human papillomaviruses (HPVs) has
hindered the study of virus-neutralizing antibodies and infection. We developed a cell-free …