Abstract The 2.1‐Å resolution crystal structure of the MATα2 homeodomain bound to DNA
reveals the unexpected presence of two nonspecifically bound α2 homeodomains, in …

The MATa homeodomain regulates the expression of cell type-specific genes in yeast. We
have determined the 2.7 A resolution crystal structure of the a2 homeodomain bound to a …

Homeodomains are a class of DNA-binding protein domains which play an important role in
genetic regulation in eukaryotes. We have characterized the thermodynamics of folding and …

The mating type homeodomain proteins, MAT a 1 and MATα2, combine to form a
heterodimer to bind DNA in diploid yeast cells. The a 1− α2 heterodimer tightly and …

Homeodomain proteins are a highly conserved class of DNA-binding proteins that are found
in virtually every eukaryotic organism. The conserved mechanism that these proteins use to …

We have engineered enhanced DNA-binding function into the a1 homeodomain by making
changes in a loop distant from the DNA-binding surface. Comparison of the free and bound …

The homeodomain proteins form a superfamily of DNA-binding proteins that play a central
role in the regulation of eukaryotic mRNA transcription. First identified as a highly conserved …

Homeodomains are helix− turn− helix type DNA-binding domains that exhibit sequence-
specific DNA binding by insertion of their “recognition” α helices into the major groove and a …

The crystal structure of the heterodimer formed by the DNA binding domains of the yeast
mating type transcription factors, MATa1 and MATα2, bound to a 21 bp DNA fragment has …

Abstract The Yeast MATa1 and MATα2 are homeodomain proteins that bind DNA
cooperatively to repress transcription of cell type specific genes. The DNA affinity and …