Quantification of cell hybridoma yields with confocal microscopy and flow cytometry

M Gabrijel, U Repnik, M Kreft, S Grilc, M Jeras… - Biochemical and …, 2004 - Elsevier
The fusion of antigen presenting and cancer cells leads to the formation of hybrid cells,
which are considered a potential vaccine for treating cancer. The quality assessment of …

[HTML][HTML] Monitoring lysosomal fusion in electrofused hybridoma cells

M Gabrijel, M Kreft, R Zorec - Biochimica et Biophysica Acta (BBA) …, 2008 - Elsevier
Dendritic and tumor cells are fused to produce hybridoma cells, which are considered to be
used as cellular vaccines to treat cancer. Previous strategies for hybridoma cell production …

Color-coded live imaging of heterokaryon formation and nuclear fusion of hybridizing cancer cells

A Suetsugu, T Matsumoto, K Hasegawa… - Anticancer …, 2016 - ar.iiarjournals.org
Background/Aim: Fusion of cancer cells has been studied for over half a century. However,
the steps involved after initial fusion between cells, such as heterokaryon formation and …

New electrofusion devices for the improved generation of dendritic cell-tumour cell hybrids

J Schaper, HR Bohnenkamp, T Noll - … for Cell Products: Proceedings of the …, 2007 - Springer
Hybrids of two different cell types are of particular interest for a couple of applications (eg
hybridoma cells for the production of monoclonal antibodies). In recent years, cancer …

[HTML][HTML] A microfluidic approach towards hybridoma generation for cancer immunotherapy

YT Lu, GP Pendharkar, CH Lu, CM Chang, CH Liu - Oncotarget, 2015 - ncbi.nlm.nih.gov
Dendritic cells/tumor fusions have shown to elicit anti-cancer immunity in different cancer
types. However, the application of these vaccines for human cancer immunotherapy are …

Generation of cell hybrids via a fusogenic cell line

SC Cheong, I Blangenois, JD Franssen… - The Journal of Gene …, 2006 - Wiley Online Library
Background Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC)
have been proposed as anti‐tumour vaccines because of their potential to combine the …

A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion

J Li, X Yu, TE Wagner, Y Wei - Oncology Letters, 2014 - spandidos-publications.com
Although the generation of hybrid cells by cell fusion plays a significant role in biotechnology
and biomedicine, the low cell‑fusion rates and the limitation of large‑scale cell fusion for …

Electrofusion generates diverse DC-tumour cell hybrids for cancer immunotherapy

JB Weise, F Hilpert, A Heiser, T Görögh… - Anticancer …, 2004 - ar.iiarjournals.org
Background: Hybrid cells generated from dendritic cells (DC) and tumour cells provide
tumour-associated antigens (TAA) in a polyvalent mode. The present study was designed to …

[HTML][HTML] Human tumour and dendritic cell hybrids generated by electrofusion: potential for cancer vaccines

TH Scott-Taylor, R Pettengell, I Clarke, G Stuhler… - … et Biophysica Acta (BBA …, 2000 - Elsevier
Hybrid cells created by fusion of antigen presenting and tumour cells have been shown to
induce potent protective and curative anti-tumour immunity in rodent cancer models. The …

Generation of highly pure fusions of colorectal carcinoma and antigen-presenting cells

U Klier, C Maletzki, E Klar, M Linnebacher - Langenbeck's archives of …, 2010 - Springer
Purpose The induction of potent T cell responses against tumors is the goal of tumor
immunotherapy. One approach is the fusion of antigen-presenting cells (APCs) with tumor …