Background We present an overview of bacterial non-classical secretion and a prediction
method for identification of proteins following signal peptide independent secretion …

Motivation Gram-positive bacteria have developed secretion systems to transport proteins
across their cell wall, a process that plays an important role during host infection. These …

Correct protein compartmentalization is a key step for molecular function and cell viability,
and this is especially true for membrane and externalized proteins of bacteria. Recent …

Protein secretion is a key virulence mechanism of pathogenic and symbiotic bacteria, which
makes the investigation of secreted proteins ('effectors') crucial for understanding the …

We present a sequence-based method, SecretomeP, for the prediction of mammalian
secretory proteins targeted to the non-classical secretory pathway, ie proteins without an N …

Bacteria have acquired multiple systems to expose proteins on their surface, release them in
the extracellular environment or even inject them into a neighboring cell. Protein secretion …

Secretory proteins perform a variety of important “remote-control” functions for bacterial
survival in the environment. The availability of complete genome sequences has allowed us …

Signal peptides are short peptides directing newly synthesized proteins toward the secretory
pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and …

Extracellular proteins are a subject of intense interest because of their essential roles in
bacterial lifestyles. However, several terms related to secretion are used confusingly in the …

In this report, we describe an optimized system for the efficient overexpression, purification,
and refolding of secreted bacterial proteins. Candidate secreted proteins were produced …