Traditional small-molecule fluorophores are always fluorescent. This attribute can obscure
valuable information in biological experiments. Here, we report on a versatile “latent” …

Small-molecule fluorophores manifest the ability of chemistry to solve problems in biology.
As we noted in a previous review (Lavis, LD; Raines, RT ACS Chem. Biol. 2008, 3, 142 …

Fluorescent probes for sensing and imaging | Nature Methods Skip to main content Thank you
for visiting nature.com. You are using a browser version with limited support for CSS. To obtain …

Chemical fluorophores find wide use in biology to detect and visualize different phenomena.
A key advantage of small-molecule dyes is the ability to construct compounds where …

Highlights•Modern microscopy methods require fluorescent labels with increased
photostability.•The photobleaching of fluorophores often involves the first triplet-excited state …

Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent
proteins. The increased demands of modern microscopy techniques on the “photon budget” …

The spatiotemporal fluorescence imaging of biological processes requires effective tools to
label intracellular biomolecules in living systems. This review presents a brief overview of …

The visualization of biologically relevant molecules and activities inside living cells
continues to transform cell biology into a truly quantitative science. However, despite the …

Small molecule fluorophores are essential tools for chemical biology. A benefit of synthetic
dyes is the ability to employ chemical approaches to control the properties and direct the …

A key current challenge in biological research is the elucidation of the that roles chemicals
and chemical reactions play in cellular function and dysfunction. Of the available cellular …