Antisense strategy represents a promising molecular tool for efficient and selective
chemotherapeutic action. It belongs among oligonucleotide strategies that employ specific …

Synthetic, chemically modified antisense oligodeoxynucleotides inhibit gene expression in a
sequence-specific manner, besides having other, sequence-non-specific effects on cells. In …

Fluorescence micro-imaging and homodyne phase-resolved confocal
microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into …

A multifrequency phase/modulation method has been developed for our UV confocal laser
microspectrofluorimeter (modulation frequency 1–200 MHz) for fluorescence lifetime …

Interaction, ie, cellular uptake and intracellular distribution, of synthetic modified antisense
oligonucleotide with the B16 melanoma cell line was studied using cationic polyene …

Short single-stranded oligonucleotides represent a class of promising therapeutics with
diverse application areas. Antisense oligonucleotides, for example, can interfere with …

The antisense therapeutic strategy makes the assumption that sequence-specific
hybridization of an oligonucleotide to its target can take place in living cells. The present …

Antisense oligomers constitute an attractive class of specific tools for genetic analysis and
for potential therapeutic applications. Targets with different cellular locations have been …

The detection of single mRNA molecules tagged by microinjected, singly fluorescently
labeled oligo (dT) 43-mer molecules in living cells in quasi-natural surrounding, that is, cell …

Uniformly sized (50 μm) porous glycidyl methacrylate/ethylene dimethacrylate particles
(SINTEF) were used as the solid phase in a sandwich type mixed-phase hybridization assay …