Ocular proteomics with emphasis on two-dimensional gel electrophoresis and mass spectrometry

N Mandal, S Heegaard, JU Prause, B Honoré… - Biological procedures …, 2010 - Springer
The intention of this review is to provide an overview of current methodologies employed in
the rapidly developing field of ocular proteomics with emphasis on sample preparation, two …

Proteomic analysis of ophthalmic disease

NL Jay, M Gillies - Clinical & experimental ophthalmology, 2012 - Wiley Online Library
Proteomics, a highly sophisticated way to study the protein profile of various biological
tissues or fluids, has hitherto had a relatively limited role ophthalmic science. Of the few …

Application of proteomic technology in eye research: a mini review

TC Lam, RKM Chun, K Li, C To - Clinical and Experimental …, 2008 - Taylor & Francis
Proteomics is a rapidly growing research area for the study of the protein cognate of
genomic data. This review gives a brief overview of the modern proteomic technology. In …

The impact of mass spectrometry application to screen new proteomics biomarkers in Ophthalmology

BNL Coronado, FBS da Cunha… - International …, 2021 - Springer
Introduction In the search for molecular markers that aid in the early diagnosis and treatment
of various human diseases, many studies have focused on changes in genes, their …

The use of proteomics in ophthalmic research

HT Steely Jr, AF Clark - Pharmacogenomics, 2000 - Taylor & Francis
The goal of molecular ophthalmology is the early detection and therapeutic treatment of eye
disease. Genomic technologies have profoundly enhanced the discovery of ocular disease …

The Human Eye Proteome Project: perspectives on an emerging proteome

RD Semba, JJ Enghild, V Venkatraman… - …, 2013 - Wiley Online Library
There are an estimated 285 million people with visual impairment worldwide, of whom 39
million are blind. The pathogenesis of many eye diseases remains poorly understood. The …

Proteomics in ocular fluids

FH Grus, SC Joachim, N Pfeiffer - PROTEOMICS–Clinical …, 2007 - Wiley Online Library
The focus of this article is to review recent techniques in proteomic analysis of ocular fluids.
These fluids include tears, aqueous humor, and vitreous, they will also be compared to …

Proteomics as a research tool in clinical and experimental ophthalmology

LM Cryan, C O'Brien - PROTEOMICS–Clinical Applications, 2008 - Wiley Online Library
It is estimated that 37 million people worldwide suffer from blindness and 124 million people
have impaired vision. While the relatively recently developed therapies, antivascular …

[33] Ocular proteomics: Cataloging photoreceptor proteins by two-dimensional gel electrophoresis and mass spectrometry

H Matsumoto, N Komori - Methods in Enzymology, 2000 - Elsevier
Publisher Summary Two-dimensional (2-D) gel electrophoresis, invented more than two
decades ago, is a powerful technique for isolating proteins from a complex biological …

[HTML][HTML] Review of application of mass spectrometry for analyses of anterior eye proteome

S Elsobky, AM Crane, M Margolis… - World journal of …, 2014 - ncbi.nlm.nih.gov
Proteins have important functional roles in the body, which can be altered in disease states.
The eye is a complex organ rich in proteins; in particular, the anterior eye is very …