Special problems require special solutions. That is why, despite the trend towards
standardization of the procedures in proteomic investigations, a number of alternative …

Abstract After IEF-IPG (see Chapters 21–24), the IPG gel strips are equilibrated in the
presence of SDS, DTT, urea, glycerol, and iodoacetamide, and placed onto the surface of a …

Abstract Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

The 2DE is a powerful proteomic technique, with excellent protein separation capabilities
where intact proteins are spatially separated by pI and molecular weight. 2DE is commonly …

Here, approaches that allow disclosure of the information hidden inside and outside of two-
dimensional gel electrophoresis (2DE) are described. Experimental identification methods …

Summary Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry is currently the workhorse for the …

Compared to classical two-dimensional electrophoresis (2-DE) with carrier ampholytes (1,
2), the advent of 2-DE with immobilized pH gradients (IPG-Dalt)(3) has produced significant …

The solubilization process for 2-D electrophoresis has to achieve four parallel goals: 1.
Breaking macromolecular interactions in order to yield separate polypeptide chains: This …

In the 1990s much of the technology development for 2-D gels was centred on attempts to
solubilise and separate whole proteomes. In the last 10 years, much has changed in the …

Abstract One-dimensional (1-D) or two-dimensional (2-D) polyacrylamide gel
electrophoresis is a convenient technique for purifying small amounts of proteins from very …