Objectives Until recently, PCR had been used to detect but not quantify Treponema
pallidum. To understand infection kinetics of this uncultivable organism, a real-time PCR …

Background Early diagnosis of Treponema pallidum infection is helpful for disease
management, and conventional PCR is suitable for lesion swabs of patients with probable …

ABSTRACT Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary
and secondary syphilis, but there is no recommendation regarding the best choice of target …

Syphilis, caused by Treponema pallidum, is a sexually transmitted disease which was
epidemic in Europe between the 15th and 20th century. From 2000 onwards it is worldwide …

The aim of this work was to investigate the application of the nested PCR assay for the
detection of Treponema pallidum (TP) DNA from the blood of patients with different stages of …

Objectives: To investigate the contribution of a real-time PCR assay for the detection of
Treponema pallidum in various biological specimens with the secondary objective of …

ABSTRACT A real-time PCR assay with a Taqman probe was developed that targeted the
polA gene of Treponema pallidum. The test was validated using an analytical panel (n= 140) …

Treponema pallidum can invade any organ, and T. pallidum DNA can be detected in various
tissues and fluids. However, the knowledge of the presence and loads of T. pallidum DNA in …

Syphilis diagnosis is based on clinical observation, serological analysis, and dark-field
microscopy (DFM) detection of Treponema pallidum subsp. pallidum, the etiological agent of …

There has been a resurgence of syphilis diagnoses in Australia. We investigated whether
our Treponema pallidum PCR test provides any additional diagnostic information over …