植物实时荧光定量PCR 内参基因的特点及选择

袁伟, 万红建, 杨悦俭 - 植物学报, 2012 - chinbullbotany.com
实时荧光定量PCR (qRT-PCR) 具有灵敏度高, 特异性强, 重复的动态定量范围和高通量等优点,
是进行植物基因表达和转录分析最常用的技术手段之一. 选择合适的内参基因是正确运用实时 …

Characterization and selection of reference genes for real-time quantitative RT-PCR of plants

W Yuan, H Wan, Y Yang - Chinese Bulletin of Botany, 2012 - chinbullbotany.com
Real-time quantitative RT-PCR (qRT-PCR) is one of the most common technologies used for
gene expression and transcriptome analysis, with its high sensitivity, specificity, good …

植物实时荧光定量PCR 内参基因的选择

胡瑞波, 范成明, 傅永福 - 中国农业科技导报, 2009 - cqvip.com
实时荧光定量RT-PCR (real-tim e quantitative reverse transcription PCR, qRT-PCR)
具有定量准确, 灵敏度高和高通量等特点, 已被广泛应用于基因的表达分析. 常规qRT-PCR …

茶树实时荧光定量PCR 分析中内参基因的选择

孙美莲, 王云生, 杨冬青, 韦朝领, 高丽萍, 夏涛… - 植物学报, 2010 - chinbullbotany.com
选择合适的内参基因是提高实时荧光定量PCR 分析(qRT-PCR) 准确性的先决条件.
该文以茶树(Camellia sinensis) 芽, 叶, 幼根, 嫩茎, 花瓣, 种子和愈伤组织为材料 …

Selection and validation of reference genes for quantitative RT-PCR analysis of gene expression in Populus trichocarpa

X Su, B Fan, L Yuan, X Cui, S Lu - Chinese Bulletin of Botany, 2013 - chinbullbotany.com
Quantitative RT-PCR (qRT-PCR) has been widely used in gene expression analysis
because of its sensitivity, specificity, and reproducibility. Application of suitable reference …

[HTML][HTML] Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze)

X Hao, DP Horvath, WS Chao, Y Yang, X Wang… - International journal of …, 2014 - mdpi.com
Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

[PDF][PDF] 柳枝稷根组织实时定量PCR 分析中内参基因的选择

蒋晓梅, 张新全, 严海东, 张瑜, 杨盛婷… - 农业生物技术 …, 2014 - journal05.magtech.org.cn
摘要选择表达稳定性高的内参基因是提高实时荧光定量PCR 分析(qRT-PCR)
准确性的重要条件. 本研究以能源植物柳枝稷(Panicum virgatum L.) 根组织为材料 …

实时荧光定量PCR 分析中毛果杨内参基因的筛选和验证

苏晓娟, 樊保国, 袁丽钗, 崔秀娜, 卢善发 - 植物学报, 2013 - chinbullbotany.com
实时荧光定量PCR (qRT-PCR) 技术具有高灵敏性, 高保真性和高特异性,
被广泛应用于基因表达的分析. 在数据处理过程中, 选用稳定表达的基因作为内参基因对准确 …

[PDF][PDF] 牡丹实时定量PCR 分析中内参基因的选择

王彦杰, 董丽, 张超王晓庆 - 农业生物技术学报, 2012 - journal05.magtech.org.cn
摘要实时荧光定量PCR (qPCR) 是目前研究基因定量表达的重要方法, 根据特定实验材料及条件
选择qPCR 分析中合适的内参基因对于准确校正目的基因的表达至关重要 …

Reference genes for real-time fluorescence quantitative PCR in Camellia sinensis

M Sun, Y Wang, D Yang, C Wei, L Gao… - Chinese Bulletin of …, 2010 - chinbullbotany.com
The selection of a suitable reference gene is an important prerequisite for successful gene
expression analysis by real-time fluorescence quantitative PCR (qPCR). We investigated the …