The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

K Kamo, AY Kim, SH Park, YH Joung - BMC Plant Biology, 2012 - Springer
Background There are many non-cereal monocots of agronomic, horticultural, and biofuel
importance. Successful transformation of these species requires an understanding of factors …

Expression of a polyubiquitin promoter isolated from Gladiolus

YH Joung, K Kamo - Plant cell reports, 2006 - Springer
A polyubiquitin promoter (GUBQ1) including its 5′ UTR and intron was isolated from the
floral monocot Gladiolus because high levels of expression could not be obtained using …

[PDF][PDF] GUS expression in Gladiolus plants controlled by two Gladiolus ubiquitin promoters

K Kamo, YH Joung, K Green - Floricult. Ornament. Biotechnol, 2009 - academia.edu
Ubiquitin represents a conserved family of genes that is involved in many metabolic
processes. The most commonly used promoter for genetic engineering of cereal monocots is …

Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment

S Salgueiro, C Pignocchi, MAJ Parry - Plant Molecular Biology, 2000 - Springer
The promoterless maize ubiquitin first exon and intron fragment can drive gusA expression
in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays …

The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression

SR Norris, SE Meyer, J Callis - Plant molecular biology, 1993 - Springer
We have isolated and determined DNA sequence for the 5′-flanking regions of three
Arabidopsis thaliana polyubiquitin genes, UBQ3, UBQ10, and UBQ11. Comparison to cDNA …

Synthetic introns help identify sequences in the 5′ UTR intron of the Glycine max polyubiquitin (Gmubi) promoter that give increased promoter activity

TNL Grant, CM De La Torre, N Zhang, JJ Finer - Planta, 2017 - Springer
Abstract Main Conclusion Specific sequences within the leader intron of a soybean
polyubiquitin gene stimulated gene expression when placed either within a synthetic intron …

Intron-mediated enhancement of gene expression in maize (Zea mays L.) and bluegrass (Poa pratensis L.)

P Vain, KR Finer, DE Engler, RC Pratt, JJ Finer - Plant cell reports, 1996 - Springer
We report a strength comparison of a large variety of monocot and dicot intron-containing
fragments inserted in the 5′ untranslated leader, between the CaMV 35S promoter and the …

Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

CW Sun, J Callis - The Plant Journal, 1997 - Wiley Online Library
The highly conserved protein ubiquitin is encoded by five polyubiquitin genes in Arabidopsis
thaliana ecotype Columbia that have been divided into two subtypes, the UBQ3/UBQ4 …

Differential expression of sugarcane polyubiquitin genes and isolation of promoters from two highly-expressed members of the gene family

H Wei, HH Albert, PH Moore - Journal of plant physiology, 1999 - Elsevier
To identify potential strong constitutive promoters, accumulation of polyubiquitin mRNA in
sugarcane (Saccharum hybrid) was studied using gene-specific probes from five sugarcane …

The intron and 5′ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues

CM De La Torre, JJ Finer - Plant cell reports, 2015 - Springer
Key message An extended version of an intron-containing soybean polyubiquitin promoter
gave very high levels of gene expression using three different validation tools. Abstract The …