Evaluation of candidate reference genes for qPCR in maize

A Manoli, A Sturaro, S Trevisan, S Quaggiotti… - Journal of plant …, 2012 - Elsevier
Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

Validation of potential reference genes for qPCR in maize across abiotic stresses, hormone treatments, and tissue types

Y Lin, C Zhang, H Lan, S Gao, H Liu, J Liu, M Cao… - PloS one, 2014 - journals.plos.org
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a powerful
and widely used technique for the measurement of gene expression. Reference genes …

Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions

Q Wan, S Chen, Z Shan, Z Yang, L Chen, C Zhang… - PloS one, 2017 - journals.plos.org
Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to
quantify gene expression. To achieve a reliable result, appropriate reference genes are …

Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development

X Han, M Lu, Y Chen, Z Zhan, Q Cui, Y Wang - 2012 - journals.plos.org
Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Buckwheat (Fagopyrum esculentum) Based on Transcriptome Sequence Data

NV Demidenko, MD Logacheva, AA Penin - PloS one, 2011 - journals.plos.org
Quantitative reverse transcription PCR (qRT-PCR) is one of the most precise and widely
used methods of gene expression analysis. A necessary prerequisite of exact and reliable …

Identification and testing of superior reference genes for a starting pool of transcript normalization in Arabidopsis

SM Hong, SC Bahn, A Lyu, HS Jung… - Plant and Cell …, 2010 - academic.oup.com
Genes that are stably expressed during development or in response to environmental
changes are essential for accurate normalization in qRT–PCR experiments. To prevent …

Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze)

X Hao, DP Horvath, WS Chao, Y Yang, X Wang… - International journal of …, 2014 - mdpi.com
Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

R Hu, C Fan, H Li, Q Zhang, YF Fu - BMC molecular biology, 2009 - Springer
Background Real-time quantitative reverse transcription PCR (RT-qPCR) data needs to be
normalized for its proper interpretation. Housekeeping genes are routinely employed for this …

Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber

H Wan, Z Zhao, C Qian, Y Sui, AA Malik, J Chen - Analytical biochemistry, 2010 - Elsevier
Quantitative real-time polymerase chain reaction (QRT-PCR) has become one of the most
widely used methods for gene expression analysis. However, the expression profile of a …

Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR

X Chi, R Hu, Q Yang, X Zhang, L Pan, N Chen… - Molecular Genetics and …, 2012 - Springer
Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for
quantifying gene expression, depends on the stability of the reference gene (s) used for data …