An improved CTAB–ammonium acetate method for total RNA isolation from cotton

L Zhao, Q Ding, J Zeng, FR Wang, J Zhang… - Phytochemical …, 2012 - Wiley Online Library
Introduction Cotton is an important economic crop. Genetic, developmental and molecular
studies of cotton require high‐quality total RNA from different tissues. Due to the richness in …

A quick and easy method for isolating good-quality RNA from cotton (Gossypium hirsutum L.) tissues

Y Wu, DJ Llewellyn, ES Dennis - Plant Molecular Biology Reporter, 2002 - Springer
Conventional RNA extraction methods have been shown to produce poor quality RNA with
low yields from cotton tissues. We present a protocol for quick isolation of cotton RNA with …

A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.)

CY Wan, TA Wilkins - Analytical biochemistry, 1994 - Elsevier
The isolation of biologically active RNA from cotton (Gossypium hirsutum L.) is difficult due to
interference by high levels of endogenous phenolics, polysaccharides, and secondary …

Effect of lyophilization of cotton tissue on quality of extractable DNA, RNA, and protein

S Saha, FE Callahan, DA Dollar, JB Creech - J Cotton Sci, 1997 - ars.usda.gov
Isolation of high quality of protein, RNA and DNA are difficult in cotton plants due to
presence of high levels of phenolic compounds. Some methods are available currently for …

A mini-scale hot borate method for the isolation of total RNA from a large number of cotton tissue samples

M Pang, JMD Stewart, J Zhang - African Journal of Biotechnology, 2011 - ajol.info
Genetic genomics in cotton requires isolating RNA from a large number of plant tissue
samples, in a mapping population. However, traditional methods for the extraction of RNA …

[HTML][HTML] A modified protocol for rapid DNA isolation from cotton (Gossypium spp.)

Q Ali, IB Salisu, A Raza, AA Shahid, AQ Rao… - MethodsX, 2019 - Elsevier
Extraction of high-quality DNA from Gossypium (Cotton) species is notoriously difficult due to
high contents of polysaccharides, quinones and polyphenols other secondary metabolites …

An improved and reproducible protocol for the extraction of high quality fungal RNA from plant biomass substrates

A Patyshakuliyeva, MR Mäkelä, OM Sietiö… - Fungal Genetics and …, 2014 - Elsevier
Isolation of high quantity and quality RNA is a crucial step in the detection of meaningful
gene expression data. Obtaining intact fungal RNA from complex lignocellulosic substrates …

Mini-scale genomic DNA extraction from cotton

B Chaudhry, A Yasmin, T Husnain… - Plant Molecular Biology …, 1999 - Springer
Large amounts of polyphenolics in cotton leaves make it difficult to obtain high-quality
genomic DNA during extraction. A procedure to isolate nuclear DNA from local cotton leaves …

RNA extraction from plant tissue with homemade acid guanidinium thiocyanate phenol chloroform (AGPC)

B Zepeda, JC Verdonk - Current Protocols, 2022 - Wiley Online Library
Gene expression studies are a powerful technique to study biological processes, and
isolating RNA that is pure, intact, and in sufficient amounts for downstream applications is …

High quality RNA isolation from ployphenol-, polysaccharide- and protein-rich tissues of lentil (Lens culinaris)

PK Dash - 3 Biotech, 2013 - Springer
Current RNA isolation methods have limitations in their ability to yield good quality and
quantity of RNA from plants that have high content of phenols, polysaccharides and storage …