Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …

Real-time quantitative polymerase chain reaction (qPCR) of complementary DNA is now a
standard method for studies of gene expression. However, qPCR can identify genuine …

Quantitative reverse transcription PCR (qRT-PCR) is one of the most precise and widely
used methods of gene expression analysis. A necessary prerequisite of exact and reliable …

Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for
quantifying gene expression, depends on the stability of the reference gene (s) used for data …

Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Genes that are stably expressed during development or in response to environmental
changes are essential for accurate normalization in qRT–PCR experiments. To prevent …

Quantifying gene expression levels is an important research tool to understand biological
systems. Reverse transcription–quantitative real-time PCR (RT–qPCR) is the preferred …

Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

Real-time RT-PCR is a powerful technique for the measurement of gene expression, but its
accuracy depends on the stability of the internal reference gene (s) used for data …