Gene expression analysis by reverse transcriptase real-time or quantitative polymerase
chain reaction (RT-qPCR) is becoming widely used for non-model plant species. Given the …

Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique for
quantifying gene expression, but its success depends on the stability of the reference gene …

Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

Mulberry (Morus alba L.) is an important economic tree species in many countries.
Quantitative real time PCR (qRT-PCR) has become a widely used method for gene …

Despite the unshakable status of reverse transcription-quantitative PCR in gene expression
analysis, it has certain disadvantages, including that the results are highly dependent on the …

Studies of gene expression are very important for the identification of genes that participate
in different biological processes. Currently, reverse transcription quantitative real‐time PCR …

Melon (Cucumis melo. L) is not only an economically important cucurbitaceous crop but also
an attractive model for studying many biological characteristics. Screening appropriate …

Reverse transcription quantitative real-time polymerase chain reaction (real-time PCR, also
referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput …

RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …

We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR)
analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the …