The need for quantitative molecular methods is growing in environmental, food, and medical
fields but is hindered by low and variable DNA extraction and by co-extraction of PCR …

Real-time quantitative PCR (qPCR) is increasingly being used for ambient water monitoring,
but development of digital polymerase chain reaction (digital PCR) has the potential to …

Environmental DNA (eDNA) has been widely used for species surveillance. However, the
lack of adequate quality control in many eDNA research projects and applications can lead …

Considerable promise and excitement exist in the application of environmental DNA (eDNA)
methods to environmental monitoring and species inventories as eDNA can provide cost …

Real-time PCR has been widely used to evaluate gene abundance in natural microbial
habitats. However, PCR-inhibitory substances often reduce the efficiency of PCR, leading to …

Isolation of environmental DNA (eDNA) is an increasingly common method for detecting
presence and assessing relative abundance of rare or elusive species in aquatic systems …

Abstract Background Environmental DNA (eDNA) analysis is increasingly being used to
detect the presence and relative abundance of rare species, especially invasive or imperiled …

Molecular‐based monitoring relying on environmental DNA (eDNA) detection became
routinely used around the world in the last few years, especially in aquatic environments …

Environmental DNA (eDNA) metabarcoding has shown great promise as an effective, non‐
invasive monitoring method for marine biomes. However, long filtration times and the need …

Quantitative PCR (qPCR) is one of the most common techniques for quantification of nucleic
acid molecules in biological and environmental samples. Although the methodology is …