Validation of a sandwich hybridization assay for marine copepod detection
JBJ Harvey, AF Djunaedi, RC Vrijenhoek - Journal of experimental marine …, 2013 - Elsevier
The sandwich hybridization assay (SHA) is a rapid and robust RNA detection method for
identifying and quantifying planktonic organisms in marine samples. Although tests for …
identifying and quantifying planktonic organisms in marine samples. Although tests for …
A 96-well plate format for detection of marine zooplankton with the sandwich hybridization assay
JBJ Harvey - Developmental biology of the sea urchin and other …, 2014 - Springer
The sandwich hybridization assay (SHA) is a ribosomal RNA (rRNA) targeted molecular
method used to detect specific target organisms from diverse communities found in …
method used to detect specific target organisms from diverse communities found in …
An improved method for achieving high-quality RNA for copepod transcriptomic studies
An efficient RNA-extraction method is crucial for transcriptomic studies of ecologically
important organisms like copepods. In this study, we used the copepod Acartia hudsonica as …
important organisms like copepods. In this study, we used the copepod Acartia hudsonica as …
Nucleic acid ratio as a proxy for starvation of coastal and pelagic copepods in the North Pacific Ocean
T Kobari, S Miyake, WT Peterson… - Plankton and Benthos …, 2017 - jstage.jst.go.jp
We investigated the nucleic acid ratio (RNA/DNA) as a proxy for starvation in copepods
collected from coastal and pelagic sites across the North Pacific Ocean. While RNA/DNA …
collected from coastal and pelagic sites across the North Pacific Ocean. While RNA/DNA …
Quantitative PCR to estimate copepod feeding
JC Nejstgaard, ME Frischer, P Simonelli, C Troedsson… - Marine Biology, 2008 - Springer
Copepods play a central role in marine food webs as grazers of plankton and as key prey for
many predators. Therefore, quantifying their specific trophic interactions is critical for …
many predators. Therefore, quantifying their specific trophic interactions is critical for …
[图书][B] Use of RNA: DNA ratios for assessing secondary production of planktonic food webs: effects of temperature, salinity, food and heavy metals
CL Speekmann - 2005 - search.proquest.com
Copepods, the dominant component of the zooplankton community, function as a vital
ecological link within marine food webs. This dissertation evaluates using RNA: DNA …
ecological link within marine food webs. This dissertation evaluates using RNA: DNA …
Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR
MJ Jungbluth, E Goetze, PH Lenz - Marine Biology, 2013 - Springer
Copepod nauplii are important in plankton food web dynamics, but limited information is
available about their ecology due to methodological challenges. Reported here is a new …
available about their ecology due to methodological challenges. Reported here is a new …
Robotic sampling, in situ monitoring and molecular detection of marine zooplankton
JBJ Harvey, JP Ryan, R Marin III, CM Preston… - Journal of Experimental …, 2012 - Elsevier
Recent advances in robotic technologies provide new opportunities to conduct high-
resolution sampling of patchily distributed zooplankton and associated environmental …
resolution sampling of patchily distributed zooplankton and associated environmental …
Does female RNA content reflect viable egg production in copepods? A test with the Baltic copepod Acartia tonsa
H Hogfors, T Holmborn, S Hajdu… - Journal of plankton …, 2011 - academic.oup.com
Biomarkers are very useful for in situ assessments of zooplankton growth. In particular, RNA-
based methods have been developed to estimate egg production in copepods. However …
based methods have been developed to estimate egg production in copepods. However …
[PDF][PDF] Toxic algal detection using rRNA-targeted probes in a semi-automated sandwich hybridization format
R Marin III, CA Scholin - Microscopic …, 2010 - repository.oceanbestpractices.org
The Sandwich Hybridisation Assay (SHA) provides a simple and rapid means to detect and
estimate cell density of a variety of algal species associated with harmful algal blooms …
estimate cell density of a variety of algal species associated with harmful algal blooms …