Neutral protease expression and optimized conditions for the degradation of blood cells using recombinant Pichia pastoris

H Zhang, B Zhang, Y Zheng, A Shan… - International …, 2014 - Elsevier
Significant amount of blood waste is generated by the livestock industry. However, the poor
utilizability of blood cells limited the application of blood. In our experiment, to degrade …

Optimisation of the enzymatic hydrolysis of blood cells with a neutral protease

Y Zheng, Q Chen, A Shan… - BioMed Research …, 2013 - Wiley Online Library
For utilizing the blood cells (BCs) effectively, enzymatic hydrolysis was applied to produce
the enzymatically hydrolyzed blood cells (EHBCs) by using a neutral protease as a catalyst …

[PDF][PDF] Medium formulation and its optimization for increased protease production by Penicillium sp. LCJ228 and its potential in blood stain removal

V Benluvankar, SE Priya, JJ Gnanadoss - J Appl Biol Biotechnol, 2016 - academia.edu
The production of protease by Penicillium sp. LCJ228 was optimized under submerged
fermentation. Nutritional and physical factors that influence protease production were …

Characterization of a novel aspartic protease from Trichoderma asperellum for the preparation of duck blood peptides

Y Xue, Q Yan, X Li, Z Jiang - Applied Microbiology and Biotechnology, 2024 - Springer
A novel aspartic protease gene (Tapro A1) from Trichoderma asperellum was successfully
expressed in Komagataella phaffii (Pichia pastoris). Ta proA1 showed 52.8% amino acid …

Heterologous Expression of Bacillus pumilus 3–19 Protease in Pichia pastoris and Its Potential Use as a Feed Additive in Poultry Farming

DS Pudova, YA Vasilyeva, MR Sharipova - BioNanoScience, 2021 - Springer
Proteases are one of the most innovative products used to improve the efficiency of feed
additives. Due to their hydrolytic properties, they enhance the absorption of amino acids …

Simultaneous hydrolysis of various protein-rich industrial wastes by a naturally evolved protease from tannery wastewater microbiota

S Ariaeenejad, K Kavousi, ASA Mamaghani… - Science of The Total …, 2022 - Elsevier
Elimination of protein-rich waste materials is one of the vital environmental protection
requirements. Using of non-naturally occurring chemicals for their remediation properties …

Efficient Secretory Expression for Mammalian Hemoglobins in Pichia pastoris

C Li, T Zhang, Z Luo, J Zhou, J Li, J Chen, G Du… - Fermentation, 2024 - mdpi.com
Mammalian hemoglobins (HB) are a kind of heme-binding proteins that play crucial
physiological roles in various organisms. The traditional techniques employed for the …

[HTML][HTML] Extracellular protease production, optimization, and partial purification from Bacillus nakamurai PL4 and its applications

IA Shaikh, B Turakani, J Malpani, SV Goudar… - Journal of King Saud …, 2023 - Elsevier
The major goal of the study was to isolate bacteria synthesizing protease enzyme from a soil
sample taken in Dandeli, Karnataka, India. Furthermore, screening, production, and …

Purification, and characterization of detergent-compatible serine protease from Bacillus safensis strain PRN1: A sustainable alternative to hazardous chemicals in …

PR Neog, S Saini, BK Konwar - Protein Expression and Purification, 2024 - Elsevier
Owing to vast therapeutic, commercial, and industrial applications of microbial proteases
microorganisms from different sources are being explored. In this regard, the gut microbiota …

Characterization of a novel protease from Aeribacillus pallidus strain VP3 with potential biotechnological interest

S Mechri, MBE Berrouina, MO Benmrad… - International journal of …, 2017 - Elsevier
The present study investigates the purification and physico-chemical characterization of an
extracellular protease from the Aeribacillus pallidus strain VP3 previously isolated from a …