Real-time quantitative PCR is nowadays a standard method to study gene expression
variations in various samples and experimental conditions. However, to interpret results …

Cabbage (Brassica oleracea) is one of the most important vegetable crops worldwide. qRT-
PCR is a sensitive technique for gene expression studies and choosing the appropriate …

Quantitative real-time PCR (qRT-PCR) is an efficient method to estimate the gene
expression levels but the accuracy of its result largely depends on the stability of the …

Drought and low temperature are the two most significant causes of abiotic stress in
agricultural crops and, therefore, they pose considerable challenges in plant science …

Selection and validation of appropriate reference genes should be the first step to consider
in experiments based on quantitative real-time polymerase chain reaction (qRT-PCR). In this …

Carex rigescens is an ornamental turfgrass in northern China which has a relatively low
maintenance cost and robust tolerance to many adverse environmental conditions, so it …

The real time quantitative reverse transcription PCR (qRT-PCR) is becoming increasingly
important to gain insight into function of genes. Given the increased sensitivity, ease and …

Selecting suitable internal reference genes is an important prerequisite for the application of
quantitative real-time PCR (qRT-PCR). However, no systematic studies have been …

Real-time quantitative polymerase chain reaction (Real-Time PCR) is a rapid, highly
sensitive, and highly specific technique, which is widely used to determine the relative …

The process of selection and validation of reference genes is the first step in studies of gene
expression by real-time quantitative polymerase chain reaction (RT-qPCR). The genome of …