Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4-coumarate: CoA ligase specificity and redundancy

X Zhou, TB Jacobs, LJ Xue, SA Harding, CJ Tsai - New Phytologist, 2015 - JSTOR
Phytologist Letters Forum 299 mutants are expected to shed further light on the functional
redundancy of the 4CL family. An advantage of gRNA stacking is the simplicity of construct …

Towards CRISPR/Cas crops–bringing together genomics and genome editing

A Scheben, F Wolter, J Batley, H Puchta… - New …, 2017 - Wiley Online Library
With the rapid increase in the global population and the impact of climate change on
agriculture, there is a need for crops with higher yields and greater tolerance to abiotic …

[HTML][HTML] Variation in mutation spectra among CRISPR/Cas9 mutagenized poplars

E Elorriaga, AL Klocko, C Ma, SH Strauss - Frontiers in plant science, 2018 - frontiersin.org
In an effort to produce reliably contained transgenic trees, we used the CRISPR/Cas9
system to alter three genes expected to be required for normal flowering in poplar (genus …

[HTML][HTML] Genome editing in trees: from multiple repair pathways to long-term stability

WP Bewg, D Ci, CJ Tsai - Frontiers in plant science, 2018 - frontiersin.org
The CRISPR technology continues to diversify with a broadening array of applications that
touch all kingdoms of life. The simplicity, versatility and species-independent nature of the …

Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing

X Tang, Y Zhang - New Phytologist, 2023 - Wiley Online Library
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …

CRISPR-Cpf1: a new tool for plant genome editing

SSA Zaidi, MM Mahfouz, S Mansoor - Trends in plant science, 2017 - cell.com
Clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated proteins
(CRISPR-Cas), a groundbreaking genome-engineering tool, has facilitated targeted trait …

[HTML][HTML] Genome editing with CRISPR/Cas9 in Pinus radiata (D. Don)

C Poovaiah, L Phillips, B Geddes, C Reeves… - BMC Plant …, 2021 - Springer
Background To meet increasing demand for forest-based products and protect natural
forests from further deforestation requires increased productivity from planted forests …

Plant genome engineering in full bloom

J Lozano-Juste, SR Cutler - Trends in plant science, 2014 - cell.com
The recent development of tools for precise editing of user-specified sequences is rapidly
changing the landscape for plant genetics and biotechnology. It is now possible to target …

[HTML][HTML] Efficient CRISPR/Cas9-mediated targeted mutagenesis in Populus in the first generation

D Fan, T Liu, C Li, B Jiao, S Li, Y Hou, K Luo - Scientific reports, 2015 - nature.com
Recently, RNA-guided genome editing using the type II clustered regularly interspaced short
palindromic repeats (CRISPR)-associated protein (Cas) system has been applied to edit the …

Duplication of a domestication locus neutralized a cryptic variant that caused a breeding barrier in tomato

S Soyk, ZH Lemmon, FJ Sedlazeck… - Nature plants, 2019 - nature.com
Genome editing technologies are being widely adopted in plant breeding. However, a
looming challenge of engineering desirable genetic variation in diverse genotypes is poor …