Bench scale synthesis of p-hydroxybenzoic acid using whole-cell nitrilase of Gordonia terrae mutant E9

V Kumar, V Kumar, N Thakur, TC Bhalla - Bioprocess and biosystems …, 2015 - Springer
Mutants of Gordonia terrae were generated using chemical mutagens for better activity,
stability and higher substrate/product tolerance of its nitrilase enzyme. Mutant E9 showed …

Transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid using nitrilase activity of Gordonia terrae

V Kumar, TC Bhalla - Biocatalysis and Biotransformation, 2013 - Taylor & Francis
Resting cells of Gordonia terrae with nitrilase (EC 3.5. 5.1) activity were investigated for
transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid. The maximum conversion …

Synthesis of vanillic acid using whole cell nitrilase of wild and mutant Gordonia terrae

TC Bhalla, Prashant, N Kumari, V Kumar… - Bioprocess and …, 2016 - Springer
The resting cells of Gordonia terrae mutant E9 having enhanced nitrilase activity were used
for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum …

Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis

A Banerjee, P Kaul, UC Banerjee - Applied microbiology and …, 2006 - Springer
Abstract (R)-mandelic acid was produced from racemic mandelonitrile using free and
immobilized cells of Pseudomonas putida MTCC 5110 harbouring a stereoselective …

Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile

H Fan, L Chen, H Sun, H Wang, Q Liu, Y Ren, D Wei - Chemical Papers, 2017 - Springer
This study aimed at developing an efficient biotransformation process for phenylacetic acid
production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a …

Nitrile-hydrolyzing enzyme from Meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid

Q Zhang, JS Gong, TT Dong, TT Liu, H Li… - Bioprocess and …, 2017 - Springer
Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis.
Traditionally, 3-HP was produced by chemical methods and fermentation process. In this …

Bioprocess Development for the Synthesis of 4-Aminophenylacetic Acid Using Nitrilase Activity of Whole Cells of Alcaligenes faecalis MTCC 12629

N Thakur, NK Sharma, S Thakur, Monika, TC Bhalla - Catalysis Letters, 2019 - Springer
Nitrilases are important industrial enzymes to synthesize commercially useful acids from
nitriles. In the present study, an aryl specific inducible nitrilase was produced from …

Modification of nitrilase based on computer screening and efficient biosynthesis of 4‐cyanobenzoic acid

YZ Jin, JD Shen, Y Wang, HM Shao, HJ Xia… - Biotechnology …, 2024 - Wiley Online Library
cyanobenzoic acid serves as a crucial intermediate for the synthesis of various high‐value
organic compounds. The enzymatic hydrolysis of terephthalonitrile to produce 4 …

Purification, characterization and in-silico analysis of nitrilase from Gordonia terrae

V Kumar, A Seth, V Kumari, V Kumar… - Protein and Peptide …, 2015 - ingentaconnect.com
An inducible and aromatic nitrilase from Gordonia terrae was purified with a yield of 19%.
The enzyme had turnover number of 63 s-1x 10-3, Km 1.4 mM and Vmax 95 Umg-1 protein …

An improved process for synthesis of nicotinic acid using hyper induced whole cell nitrilase of Gordonia terrae MTCC8139

Monika, Sheetal, N Thakur, TC Bhalla - 3 Biotech, 2022 - Springer
Nitrilase mediated synthesis of nicotinic acid (vitamin B3) from 3-cyanopyridine has
emerged as promising viable alternative to its chemical synthesis. In the present …