Bench scale synthesis of p-hydroxybenzoic acid using whole-cell nitrilase of Gordonia terrae mutant E9
Mutants of Gordonia terrae were generated using chemical mutagens for better activity,
stability and higher substrate/product tolerance of its nitrilase enzyme. Mutant E9 showed …
stability and higher substrate/product tolerance of its nitrilase enzyme. Mutant E9 showed …
Transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid using nitrilase activity of Gordonia terrae
V Kumar, TC Bhalla - Biocatalysis and Biotransformation, 2013 - Taylor & Francis
Resting cells of Gordonia terrae with nitrilase (EC 3.5. 5.1) activity were investigated for
transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid. The maximum conversion …
transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid. The maximum conversion …
Synthesis of vanillic acid using whole cell nitrilase of wild and mutant Gordonia terrae
The resting cells of Gordonia terrae mutant E9 having enhanced nitrilase activity were used
for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum …
for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum …
Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis
A Banerjee, P Kaul, UC Banerjee - Applied microbiology and …, 2006 - Springer
Abstract (R)-mandelic acid was produced from racemic mandelonitrile using free and
immobilized cells of Pseudomonas putida MTCC 5110 harbouring a stereoselective …
immobilized cells of Pseudomonas putida MTCC 5110 harbouring a stereoselective …
Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile
H Fan, L Chen, H Sun, H Wang, Q Liu, Y Ren, D Wei - Chemical Papers, 2017 - Springer
This study aimed at developing an efficient biotransformation process for phenylacetic acid
production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a …
production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a …
Nitrile-hydrolyzing enzyme from Meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid
Q Zhang, JS Gong, TT Dong, TT Liu, H Li… - Bioprocess and …, 2017 - Springer
Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis.
Traditionally, 3-HP was produced by chemical methods and fermentation process. In this …
Traditionally, 3-HP was produced by chemical methods and fermentation process. In this …
Bioprocess Development for the Synthesis of 4-Aminophenylacetic Acid Using Nitrilase Activity of Whole Cells of Alcaligenes faecalis MTCC 12629
N Thakur, NK Sharma, S Thakur, Monika, TC Bhalla - Catalysis Letters, 2019 - Springer
Nitrilases are important industrial enzymes to synthesize commercially useful acids from
nitriles. In the present study, an aryl specific inducible nitrilase was produced from …
nitriles. In the present study, an aryl specific inducible nitrilase was produced from …
Modification of nitrilase based on computer screening and efficient biosynthesis of 4‐cyanobenzoic acid
YZ Jin, JD Shen, Y Wang, HM Shao, HJ Xia… - Biotechnology …, 2024 - Wiley Online Library
cyanobenzoic acid serves as a crucial intermediate for the synthesis of various high‐value
organic compounds. The enzymatic hydrolysis of terephthalonitrile to produce 4 …
organic compounds. The enzymatic hydrolysis of terephthalonitrile to produce 4 …
Purification, characterization and in-silico analysis of nitrilase from Gordonia terrae
An inducible and aromatic nitrilase from Gordonia terrae was purified with a yield of 19%.
The enzyme had turnover number of 63 s-1x 10-3, Km 1.4 mM and Vmax 95 Umg-1 protein …
The enzyme had turnover number of 63 s-1x 10-3, Km 1.4 mM and Vmax 95 Umg-1 protein …
An improved process for synthesis of nicotinic acid using hyper induced whole cell nitrilase of Gordonia terrae MTCC8139
Monika, Sheetal, N Thakur, TC Bhalla - 3 Biotech, 2022 - Springer
Nitrilase mediated synthesis of nicotinic acid (vitamin B3) from 3-cyanopyridine has
emerged as promising viable alternative to its chemical synthesis. In the present …
emerged as promising viable alternative to its chemical synthesis. In the present …