Fungal identification biases in microbiome projects

L Tedersoo, B Lindahl - Environmental microbiology reports, 2016 - Wiley Online Library
Fungi are the key players in ecosystems as well as in plant and human health. High‐
throughput molecular identification of fungi has greatly progressed our understanding about …

Identifying the 'unidentified'fungi: a global-scale long-read third-generation sequencing approach

L Tedersoo, S Anslan, M Bahram, U Kõljalg… - Fungal Diversity, 2020 - Springer
Molecular identification methods, in particular high-throughput sequencing tools, have
greatly improved our knowledge about fungal diversity and biogeography, but many of the …

Back to the future: natural history and the way forward in modern fungal ecology

KG Peay - Fungal Ecology, 2014 - Elsevier
The growing power and increasing availability of molecular tools for identifying fungi in
environmental samples has revolutionized the way that fungal ecologists work. As a result …

Different amplicon targets for sequencing-based studies of fungal diversity

F De Filippis, M Laiola, G Blaiotta… - Applied and …, 2017 - Am Soc Microbiol
Target-gene amplicon sequencing is the most exploited high-throughput sequencing
application in microbial ecology. The targets are taxonomically relevant genes, with 16S …

[HTML][HTML] Critical issues in mycobiota analysis

B Halwachs, N Madhusudhan, R Krause… - Frontiers in …, 2017 - frontiersin.org
Fungi constitute an important part of the human microbiota and they play a significant role for
health and disease development. Advancements made in the culture-independent analysis …

Ghost-tree: creating hybrid-gene phylogenetic trees for diversity analyses

J Fouquier, JR Rideout, E Bolyen, J Chase, A Shiffer… - Microbiome, 2016 - Springer
Background Fungi play critical roles in many ecosystems, cause serious diseases in plants
and animals, and pose significant threats to human health and structural integrity problems …

Molecular techniques for pathogen identification and fungus detection in the environment

CKM Tsui, J Woodhall, W Chen, CA Lévesque, A Lau… - IMA fungus, 2011 - Springer
Many species of fungi can cause disease in plants, animals and humans. Accurate and
robust detection and quantification of fungi is essential for diagnosis, modeling and …

Accurate estimation of fungal diversity and abundance through improved lineage-specific primers optimized for Illumina amplicon sequencing

DL Taylor, WA Walters, NJ Lennon… - Applied and …, 2016 - Am Soc Microbiol
While high-throughput sequencing methods are revolutionizing fungal ecology, recovering
accurate estimates of species richness and abundance has proven elusive. We sought to …

Sequence-based classification and identification of Fungi

D Hibbett, K Abarenkov, U Kõljalg, M Öpik, B Chai… - Mycologia, 2016 - Taylor & Francis
Fungal taxonomy and ecology have been revolutionized by the application of molecular
methods and both have increasing connections to genomics and functional biology …

Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …