Production of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue

CG Da Silva, CF Martins, TC Cardoso… - … " Cloning and Stem …, 2016 - liebertpub.com
The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are
they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells …

Bone marrow mesenchymal stem cells are an attractive donor cell type for production of cloned pigs as well as genetically modified cloned pigs by somatic cell …

Z Li, X He, L Chen, J Shi, R Zhou, W Xu… - … Formerly" Cloning and …, 2013 - liebertpub.com
The somatic cell nuclear transfer (SCNT) technique has been widely applied to clone pigs or
to produce genetically modified pigs. Currently, this technique relies mainly on using …

Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos

GA Kim, HJ Oh, TH Lee, JH Lee, SH Oh, JH Lee… - Theriogenology, 2014 - Elsevier
Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for
somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures …

Analysis of cell growth and gene expression of porcine adipose tissue‐derived mesenchymal stem cells as nuclear donor cell

HJ Oh, JE Park, EJ Park, MJ Kim… - Development …, 2014 - Wiley Online Library
In several laboratory animals and humans, adipose tissue‐derived mesenchymal stem cells
(ASC) are of considerable interest because they are easy to harvest and can generate a …

Establishment, differentiation, electroporation and nuclear transfer of porcine mesenchymal stem cells

Z Song, P Cong, Q Ji, L Chen, Y Nie… - … in Domestic Animals, 2015 - Wiley Online Library
Contents The limited success of somatic cell nuclear transfer (SCNT) is largely attributed to
defects in epigenetic reprogramming of the donor genome. Donor cell types with distinct …

[PDF][PDF] Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

H Jin, BM Kumar, J Kim, H Song, Y Jeong… - International Journal …, 2007 - researchgate.net
In the present study, we have characterized an isolated population of porcine bone marrow
mesenchymal stem cells (MSCs) for multilineage commitment and compared the …

Production of pigs by hand-made cloning using mesenchymal stem cells and fibroblasts

Z Yang, G Vajta, Y Xu, J Luan, M Lin, C Liu… - … " Cloning and Stem …, 2016 - liebertpub.com
Mesenchymal stem cells (MSCs) exhibited self-renewal and less differentiation, making the
MSCs promising candidates for adult somatic cell nuclear transfer (SCNT). In this article, we …

Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs

R Faast, SJ Harrison, LFS Beebe… - Cloning and stem …, 2006 - liebertpub.com
Mesenchymal stem cells (MSCs) isolated from bone marrow were used to examine the
hypothesis that a less differentiated cell type could increase adult somatic cell nuclear …

Effect of Embryo Aggregation on In Vitro Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones

V Savy, V Alberio, G Vans Landschoot… - Cellular …, 2021 - liebertpub.com
Somatic cell nuclear transfer (SCNT) is a method with unique ability to reprogram the
epigenome of a fully differentiated cell. However, its efficiency remains extremely low. In this …

[PDF][PDF] The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

H Nazari, A Shirazi, N Shams-Esfandabadi, A Afzali… - Int J Dev …, 2016 - academia.edu
Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a
major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to …