Screening for plant viruses by next generation sequencing using a modified double strand RNA extraction protocol with an internal amplification control
P Kesanakurti, M Belton, H Saeed, H Rast… - Journal of Virological …, 2016 - Elsevier
The majority of plant viruses contain RNA genomes. Detection of viral RNA genomes in
infected plant material by next generation sequencing (NGS) is possible through the …
infected plant material by next generation sequencing (NGS) is possible through the …
Viral double-stranded RNAs (dsRNAs) from plants: alternative nucleic acid substrates for high-throughput sequencing
A Marais, C Faure, B Bergey, T Candresse - Viral metagenomics: methods …, 2018 - Springer
High-throughput sequencing (or next-generation sequencing—NGS) is an emerging
technology that allows the detection of plant viruses without any prior knowledge. Various …
technology that allows the detection of plant viruses without any prior knowledge. Various …
A new virus discovered by immunocapture of double‐stranded RNA, a rapid method for virus enrichment in metagenomic studies
Next‐generation sequencing technologies enable the rapid identification of viral infection of
diseased organisms. However, despite a consistent decrease in sequencing costs, it is …
diseased organisms. However, despite a consistent decrease in sequencing costs, it is …
A new method to isolate total dsRNA
G Atsumi, KT Sekine, K Kobayashi - … new approaches to detect viruses and …, 2015 - Springer
When a diseased plant is suspected to be infected with unknown viruses, the approach of
isolating double-stranded RNA (dsRNA) from diseased tissues and analyzing the sequence …
isolating double-stranded RNA (dsRNA) from diseased tissues and analyzing the sequence …
Next generation sequencing for detection and discovery of plant viruses and viroids: comparison of two approaches
Next generation sequencing (NGS) technologies are becoming routinely employed in
different fields of virus research. Different sequencing platforms and sample preparation …
different fields of virus research. Different sequencing platforms and sample preparation …
A new method for extraction of double-stranded RNA from plants
IE Tzanetakis, RR Martin - Journal of Virological Methods, 2008 - Elsevier
The occurrence of high molecular weight double-stranded RNA (dsRNA) in plants is
associated with the presence of RNA viruses. DsRNA is stable, can be extracted easily from …
associated with the presence of RNA viruses. DsRNA is stable, can be extracted easily from …
Viral diagnostics in plants using next generation sequencing: computational analysis in practice
S Jones, A Baizan-Edge, S MacFarlane… - Frontiers in plant …, 2017 - frontiersin.org
Viruses cause significant yield and quality losses in a wide variety of cultivated crops.
Hence, the detection and identification of viruses is a crucial facet of successful crop …
Hence, the detection and identification of viruses is a crucial facet of successful crop …
Interlaboratory comparison study on ribodepleted total RNA high-throughput sequencing for plant virus diagnostics and bioinformatic competence
YZA Gaafar, M Westenberg, M Botermans, K László… - Pathogens, 2021 - mdpi.com
High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing
interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of …
interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of …
Virus detection by high-throughput sequencing of small RNAs: Large-scale performance testing of sequence analysis strategies
Recent developments in high-throughput sequencing (HTS), also called next-generation
sequencing (NGS), technologies and bioinformatics have drastically changed research on …
sequencing (NGS), technologies and bioinformatics have drastically changed research on …
Detection of plant viruses in natural environments by using RNA-Seq
Sequencing of RNA by next generation sequencers, RNA-Seq, is revolutionizing virus
detection. In addition to the unbiased detection of various viruses from wild plants in natural …
detection. In addition to the unbiased detection of various viruses from wild plants in natural …