Despite recent advances, our knowledge of potential and rare human pathogens is far from
exhaustive. Current molecular diagnostic tools mainly rely on the specific amplification of …

Because of the constant threat posed by emerging infectious diseases and the limitations of
existing approaches used to identify new pathogens, there is a great demand for new …

Objective To provide a feasible and cost-effective next-generation sequencing (NGS)
method for accurate identification of viral pathogens in clinical specimens, because …

Background Massive, parallel sequencing is a potent tool for dissecting the regulation of
biological processes by revealing the dynamics of the cellular RNA profile under different …

A crucial step in the molecular detection of viruses in clinical specimens is the efficient
extraction of viral nucleic acids. The total yield of viral nucleic acid from a clinical specimen …

Next-generation sequencing is a powerful tool for viral genomics. Viruses often constitute a
very small proportion of any given sample meaning that methods that enable detection of …

Viral infections cause many different diseases stemming both from well-characterized viral
pathogens but also from emerging viruses, and the search for novel viruses continues to be …

Rapid and reliable virus subtype identification is critical for accurate diagnosis of human
infections, effective response to epidemic outbreaks and global-scale surveillance of highly …

Accurate identification of biological threat agents, especially RNA viruses, in clinical or
environmental samples can be challenging because the concentration of viral genomic …

Comparing sequences of archived viruses collected over many years to the present allows
the study of viral evolution and contributes to the design of new vaccines. However, the …