Recent advances in sequencing technologies have transformed the field of virus discovery
and virome analysis. Once mostly confined to the traditional Sanger sequencing based …

Insensitivity and technical complexity have impeded the implementation of high-throughput
nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories …

Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral
populations. It is culture-independent, requires no a priori knowledge of the viruses in the …

Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral
populations. It is culture independent, requires no a priori knowledge of the viruses in the …

Highlights•New amplification methods reduce biases caused by whole genome
amplification•Biases caused by assembly can be avoided using long-read …

Abstract The advent of Next Generation Sequencing (NGS) has vastly increased our ability
to discover novel viruses and to systematically define the spectrum of viruses present in a …

Background Next generation sequencing (NGS) technology allows laboratories to
investigate virome composition in clinical and environmental samples in a culture …

Widespread in public databases, foreign contaminant sequences pose a substantial
obstacle in genomic analyses. Such contamination in viral genome databases is also …

Next-generation sequencing has opened avenues to studying complex populations such as
the bacteriome (all bacteria), mycobiome (all fungi), and virome (all viruses in a given …

Objectives Sample preparation for high-throughput sequencing (HTS) includes treatment
with various laboratory components, potentially carrying viral nucleic acids, the extent of …