Efficient oligo nucleotide mediated CRISPR-Cas9 gene editing in Aspergilli
CS Nødvig, JB Hoof, ME Kogle, ZD Jarczynska… - Fungal Genetics and …, 2018 - Elsevier
CRISPR-Cas9 technologies are revolutionizing fungal gene editing. Here we show that
survival of specific Cas9/sgRNA mediated DNA double strand breaks (DSBs) depends on …
survival of specific Cas9/sgRNA mediated DNA double strand breaks (DSBs) depends on …
[HTML][HTML] Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi
TM van Leeuwe, M Arentshorst, T Ernst, E Alazi… - Fungal biology and …, 2019 - Springer
Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing
multiple gene alterations in filamentous fungi, whereas traditional methods are time …
multiple gene alterations in filamentous fungi, whereas traditional methods are time …
[HTML][HTML] Cpf1 enables fast and efficient genome editing in Aspergilli
KG Vanegas, ZD Jarczynska, T Strucko… - Fungal Biology and …, 2019 - Springer
Background CRISPR technology has revolutionized fungal genetic engineering by
increasing the speed and complexity of the experiments that can be performed. Moreover …
increasing the speed and complexity of the experiments that can be performed. Moreover …
A Simple and Universal System for Gene Manipulation in Aspergillus fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with …
Q Al Abdallah, W Ge, JR Fortwendel - Msphere, 2017 - Am Soc Microbiol
ABSTRACT CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a
novel genome-editing system that has been successfully established in Aspergillus …
novel genome-editing system that has been successfully established in Aspergillus …
[HTML][HTML] CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
CRISPR-Cas mediated genome engineering has revolutionized functional genomics.
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
[HTML][HTML] Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger
L Song, JP Ouedraogo, M Kolbusz, TTM Nguyen… - PloS one, 2018 - journals.plos.org
As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been
applied in filamentous fungi to improve the efficiency of genome alteration. However, the …
applied in filamentous fungi to improve the efficiency of genome alteration. However, the …
[HTML][HTML] Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion
L Huang, H Dong, J Zheng, B Wang, L Pan - Microbiological research, 2019 - Elsevier
Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-
strand breaks for genome editing. To prevent the potential hazard caused by double-strand …
strand breaks for genome editing. To prevent the potential hazard caused by double-strand …
[HTML][HTML] CRISPR_Cas systems for fungal research
JP Ouedraogo, A Tsang - Fungal Biology Reviews, 2020 - Elsevier
Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have
improved our ability to precisely edit genomes and control gene expression. We summarize …
improved our ability to precisely edit genomes and control gene expression. We summarize …
[HTML][HTML] Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi
MJ Kwon, T Schütze, S Spohner, S Haefner… - Fungal biology and …, 2019 - Springer
Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …
[HTML][HTML] CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …
production. With the increasing number of full genomes available for fungal species, the …