Efficient Over-expression and Application of High-performance Pectin Lyase by Screening Aspergillus niger Pectin Lyase Gene Family

Y He, L Pan, B Wang - Biotechnology and Bioprocess Engineering, 2018 - Springer
Acidic pectin lyase is of great application value in fruit-processing industry. In this study, five
pectin lyase genes (pelA, pelB, pelC, pelD and pelF) from A. niger pectin lyase gene family …

An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification

SX Xu, X Qin, B Liu, DQ Zhang, W Zhang… - Letters in applied …, 2015 - academic.oup.com
The pectin lyase gene pnl‐zj5a from Aspergillus niger ZJ5 was identified and expressed in
Pichia pastoris. PNL‐ZJ5A was purified by ultrafiltration, anion exchange and gel …

Expression of a pectin lyase A gene from Aspergillus niger in Pichia pastoris GS115

H Qiang, X Yang, B Tian, C Ke, W Lin, R Lü… - Sheng wu gong …, 2009 - europepmc.org
In this study, the mature peptide sequence of a pectin lyase gene A was amplified from
Aspergillus niger strain EIM-6 by using RT-PCR reverse transcription technique. The cloned …

Production, purification and characterization of pectin lyase from Bacillus subtilis isolated from moong beans leaves (Vigna radiata)

R Saharan, KP Sharma - Biocatalysis and Agricultural Biotechnology, 2019 - Elsevier
Abstract Pectin lyase (EC 4.2. 2.10) is the enzyme known to act on highly esterified pectin
substances via β-elimination mechanism without producing methanol. It is potentially …

Characterization of an Alkaline Thermostable Pectin Lyase from Newly Isolated Aspergillus niger _WHAK1 and Its Application on Fruit Juice Clarification

K Poturcu, I Ozmen, HH Biyik - Arabian Journal for Science and …, 2017 - Springer
Pectin lyase degrades pectic substances that are complex polysaccharides of middle
lamella and primary cell walls of plants. In this study, alkaline thermostable pectin lyase from …

Molecular biology, genomics and bioinformatics insights into fungal pectin lyase: an overview

S Yadav, PK Yadav, AK Dubey, G Anand… - … of biotechnology for …, 2017 - Springer
Pectinase represents an industrially important group of enzymes, comprising pectin lyases
(PNL), pectate lyases (PL), polygalacturonases (PG), and pectin methylesterases (PME) as …

[HTML][HTML] Comparative Characterization of Aspergillus Pectin Lyases by Discriminative Substrate Degradation Profiling

B Zeuner, TB Thomsen, MA Stringer… - … in Bioengineering and …, 2020 - frontiersin.org
Fungal genomes often contain several copies of genes that encode carbohydrate active
enzymes having similar activity. The copies usually have slight sequence variability, and it …

Expression of pectin lyase 1 from Aspergillus oryzae in Escherichia coli

QX Zhao, S Yuan, YL Zhang - Sheng wu Gong Cheng xue bao …, 2007 - europepmc.org
Pectin lyases from Aspergillus oryzae and Aspergillus niger are usually used for the
production of traditional fermented foods, but these fungi produce less pectinases under …

Heterologous expression of a Penicillium purpurogenum pectin lyase in Pichia pastoris and its characterization

C Pérez-Fuentes, MC Ravanal, J Eyzaguirre - Fungal biology, 2014 - Elsevier
Lignocellulose is the major component of plant cell walls and it represents a great source of
renewable organic matter. One of lignocellulose constituents is pectin. Pectin is composed …

[PDF][PDF] Optimization, purification and characterization of pectinases from pectinolytic strain, Aspergillus foetid us MTCC 10559

S Kumar, NK Jain, KC Sharma, R Paswan… - Journal of …, 2015 - researchgate.net
Journal of Environmental Biology, Vol. 36, 483-489, March 2015© Triveni Enterprises,
Lucknow (India) by sodium, potassium or ammonium ions. Pectinases breakdown complex …