Gel electrophoresis-based plant proteomics: Past, present, and future. Happy 10th anniversary Journal of Proteomics!

JV Jorrin-Novo, S Komatsu, R Sanchez-Lucas… - Journal of …, 2019 - Elsevier
In this century we have assisted at an unimaginable expansion of proteomics, with
continuous innovations and optimizations in methods, techniques, protocols, equipment …

[HTML][HTML] Gel-based proteomics in plants: time to move on from the tradition

AK Anguraj Vadivel - Frontiers in plant science, 2015 - frontiersin.org
In more than 20 years of the proteomics era, considerable technical developments and
scientific discoveries have contributed to the advancement of this field of research. Gel …

Challenges and prospects of plant proteomics

KJ van Wijk - Plant Physiology, 2001 - academic.oup.com
Proteomics has been defined as “the systematic analysis of the protein population in a
tissue, cell, or subcellular compartment.” Over the last 2 to 3 years, proteomics has …

Integrated plant proteomics—putting the green genomes to work

JL Heazlewood, AH Millar - Functional Plant Biology, 2003 - CSIRO Publishing
Protein analysis has been at the heart of plant science for many years, but with new
questions emerging from an abundance of genomic information and further improvements in …

[HTML][HTML] Time to dig deep into the plant proteome: a hunt for low-abundance proteins

R Gupta, Y Wang, GK Agrawal, R Rakwal… - Frontiers in plant …, 2015 - frontiersin.org
Two-dimensional gel electrophoresis (2-DGE) has come a long way since its introduction
around 40 years by the pioneering work of these three researchers (Klose, 1975; O'Farrell …

Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 1. Assessing analytical validation

MC Ruebelt, NK Leimgruber, M Lipp… - Journal of agricultural …, 2006 - ACS Publications
Current tools used to assess the safety of food and feed derived from modern biotechnology
emphasize the investigation of possible unintended effects caused directly by the …

Proteomics for genetic and physiological studies in plants

H Thiellement, N Bahrman, C Damerval… - …, 1999 - Wiley Online Library
Proteomics is becoming a necessity in plant biology, as it is in medicine, zoology and
microbiology, for deciphering the function and role of the genes that are or will be …

Gel‐based and gel‐free quantitative proteomics approaches at a glance

C Abdallah, E Dumas-Gaudot, J Renaut… - … journal of plant …, 2012 - Wiley Online Library
Two‐dimensional gel electrophoresis (2‐DE) is widely applied and remains the method of
choice in proteomics; however, pervasive 2‐DE‐related concerns undermine its prospects …

Two-dimensional electrophoresis for plant proteomics

W Weiss, A Görg - Plant Proteomics: Methods and Protocols, 2007 - Springer
Abstract Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

Fourteen years of plant proteomics reflected in Proteomics: Moving from model species and 2DE‐based approaches to orphan species and gel‐free platforms

JV Jorrín‐Novo, J Pascual, R Sánchez‐Lucas… - …, 2015 - Wiley Online Library
In this article, the topic of plant proteomics is reviewed based on related papers published in
the journal Proteomics since publication of the first issue in 2001. In total, around 300 …