Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy
Two-photon microscopy is used to image neuronal activity, but has severe limitations for
studying deeper cortical layers. Here, we developed a custom three-photon microscope …
studying deeper cortical layers. Here, we developed a custom three-photon microscope …
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
High-resolution optical imaging is critical to understanding brain function. We demonstrate
that three-photon microscopy at 1,300-nm excitation enables functional imaging of …
that three-photon microscopy at 1,300-nm excitation enables functional imaging of …
Miniature three-photon microscopy maximized for scattered fluorescence collection
C Zhao, S Chen, L Zhang, D Zhang, R Wu, Y Hu… - Nature …, 2023 - nature.com
In deep-tissue multiphoton microscopy, diffusion and scattering of fluorescent photons,
rather than ballistic emanation from the focal point, have been a confounding factor. Here we …
rather than ballistic emanation from the focal point, have been a confounding factor. Here we …
Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain
Two-photon calcium imaging provides an optical readout of neuronal activity in populations
of neurons with subcellular resolution. However, conventional two-photon imaging systems …
of neurons with subcellular resolution. However, conventional two-photon imaging systems …
Suite2p: beyond 10,000 neurons with standard two-photon microscopy
The combination of two-photon microscopy recordings and powerful calcium-dependent
fluorescent sensors enables simultaneous recording of unprecedentedly large populations …
fluorescent sensors enables simultaneous recording of unprecedentedly large populations …
In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Two-photon fluorescence microscopy enables scientists in various fields including
neuroscience,, embryology and oncology to visualize in vivo and ex vivo tissue morphology …
neuroscience,, embryology and oncology to visualize in vivo and ex vivo tissue morphology …
Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing
Two-photon microscopy, combined with the appropriate optical labelling, enables the
measurement and tracking of submicrometer structures within brain cells, as well as the …
measurement and tracking of submicrometer structures within brain cells, as well as the …
[HTML][HTML] Chronic cellular imaging of entire cortical columns in awake mice using microprisms
ML Andermann, NB Gilfoy, GJ Goldey, RNS Sachdev… - Neuron, 2013 - cell.com
Two-photon imaging of cortical neurons in vivo has provided unique insights into the
structure, function, and plasticity of cortical networks, but this method does not currently …
structure, function, and plasticity of cortical networks, but this method does not currently …
Extended depth of field microscopy for rapid volumetric two-photon imaging
G Thériault, Y De Koninck, N McCarthy - Optics express, 2013 - opg.optica.org
Two-photon fluorescence microscopy is an influential tool in biology, providing valuable
information on the activity of cells deep inside the tissue. However, it is limited by its low …
information on the activity of cells deep inside the tissue. However, it is limited by its low …