From sequencing to genome editing for cotton improvement

R Peng, DC Jones, F Liu, B Zhang - Trends in Biotechnology, 2021 - cell.com
Traditional breeding techniques are proven, but additional knowledge learned from genome
sequencing provides vast new data that might help identify gene targets for improving cotton …

[HTML][HTML] Genome editing in cotton: challenges and opportunities

Z Khan, SH Khan, A Ahmed, MU Iqbal… - Journal of Cotton …, 2023 - Springer
Cotton has enormous economic potential providing high-quality protein, oil, and fibre. A
large increase in cotton output is necessary due to the world's changing climate and …

Application of CRISPR/Cas system for genome editing in cotton

S Fiaz, SA Khan, A Younas, K Shahzad, H Ali… - CRISPR and RNAi …, 2021 - Elsevier
Cotton holds immense economic potential owing to its premium quality fiber, oil, and protein
contents. The ever-growing human population along with a global shift in climate requires a …

[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)

B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

[HTML][HTML] Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …

A comprehensive overview of cotton genomics, biotechnology and molecular biological studies

X Wen, Z Chen, Z Yang, M Wang, S Jin, G Wang… - Science China Life …, 2023 - Springer
Cotton is an irreplaceable economic crop currently domesticated in the human world for its
extremely elongated fiber cells specialized in seed epidermis, which makes it of high …

Genome editing in cotton using CRISPR/Cas9 system

C Li, B Zhang - Transgenic Cotton: Methods and Protocols, 2019 - Springer
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system is emerging as effective strategy for generating site-specific …

[HTML][HTML] A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

C Li, T Unver, B Zhang - Scientific reports, 2017 - nature.com
The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …

CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review

A Rasheed, AA Barqawi, A Mahmood, M Nawaz… - Molecular Biology …, 2022 - Springer
Legumes are an imperative source of food and proteins across the globe. They also improve
soil fertility through symbiotic nitrogen fixation (SNF). Genome editing (GE) is now a novel …

[HTML][HTML] Genome editing in cotton with the CRISPR/Cas9 system

W Gao, L Long, X Tian, F Xu, J Liu, PK Singh… - Frontiers in plant …, 2017 - frontiersin.org
Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …