Metagenomics is a powerful tool to identify novel or unexpected pathogens, since it is
generic and relatively unbiased. The limit of detection (LOD) is a critical parameter for the …

Clinical metagenomics is a broad-range agnostic detection method of pathogens, including
novel microorganisms. A major limit is the low pathogen load compared to the high …

Shotgun metagenomics using next generation sequencing (NGS) is a promising technique
to analyze both DNA and RNA microbial material from patient samples. Mostly used in a …

Introduction Metagenomic sequencing is increasingly being used in clinical settings for
difficult to diagnose cases. The performance of viral metagenomic protocols relies to a large …

Metagenomics is gradually being implemented for diagnosing infectious diseases. However,
in-depth protocol comparisons for viral detection have been limited to individual sets of …

We describe the most common internal and external sources and types of contamination
encountered in viral metagenomic studies and discuss their negative impact on sequencing …

Viral enrichment by probe hybridization has been reported to significantly increase the
sensitivity of viral metagenomics. This study compares the analytical performance of two …

Infection in the central nervous system is a severe condition associated with high morbidity
and mortality. Despite ample testing, the majority of encephalitis and meningitis cases …

Metagenomic Next Generation Sequencing (mNGS) allows the evaluation of complex
microbial communities, avoiding isolation and cultivation of each microbial species, and …

Metagenomic next-generation sequencing (mNGS) is an untargeted technique for
determination of microbial DNA/RNA sequences in a variety of sample types from patients …