This review aims to assess and recommend approaches for targeted and agnostic High
Throughput Sequencing of RNA viruses in a variety of sample matrices. HTS also referred to …

Individual RNA viruses typically occur as populations of genomes that differ slightly from
each other due to mutations introduced by the error-prone viral polymerase. Understanding …

In the face of numerous emerging and re-emerging viral threats, large-scale genome
sequencing efforts are underway to monitor viral evolution in real-time. To fully appreciate …

High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing
interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of …

Background The use of next-generation sequencing has become an established method for
virus detection. Efficient study design for accurate detection relies on the optimal amount of …

Several biosafety level 3 and/or 4 (BSL-3/4) pathogens are high-consequence, single-
stranded RNA viruses, and their genomes, when introduced into permissive cells, are …

A key step for broad viral detection using high-throughput sequencing (HTS) is optimizing
the sample preparation strategy for extracting viral-specific nucleic acids since viral …

Detection of viruses in the environment is heavily dependent on PCR-based approaches
that require reference sequences for primer design. While this strategy can accurately detect …

Extraction of viral RNA and the storage of sample material are extremely important factors in
the detection and whole genome sequencing (WGS) of viral pathogens. Although PCR …

Comparing sequences of archived viruses collected over many years to the present allows
the study of viral evolution and contributes to the design of new vaccines. However, the …