Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment

BB Zhang, X Shen, XJ Li, YB Tian, HJ Ouyang… - Scientific Reports, 2021 - nature.com
In quantitative PCR research, appropriate reference genes are key to determining accurate
mRNA expression levels. In order to screen the reference genes suitable for detecting gene …

Validation of Reference Genes via qRT-PCR in Multiple Conditions in Brandt's Voles, Lasiopodomys brandtii

L Tian, Y Chen, DW Wang, XH Liu - Animals, 2021 - mdpi.com
Simple Summary This study validated the stability of the expression profiles of nine common
candidate reference genes (Gapdh, Hprt1, β-actin, PPIA, Rpl13a, Tbp, Sdha, Hmbs, and …

[HTML][HTML] Selection of reliable reference genes for real-time qRT-PCR analysis of Zi geese (Anser anser domestica) gene expression

H Ji, J Wang, J Liu, J Guo, Z Wang… - … journal of animal …, 2013 - ncbi.nlm.nih.gov
Zi geese (Anser anser domestica) belong to the white geese and are excellent layers with a
superior feed-to-egg conversion ratio. Quantitative gene expression analysis, such as Real …

Reference gene validation for quantification of gene expression during ovarian development of turbot (Scophthalmus maximus)

Y Gao, Y Gao, B Huang, Z Meng, Y Jia - Scientific Reports, 2020 - nature.com
Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a
powerful and sensitive method used in gene expression analysis. Suitable reference genes …

[HTML][HTML] Reference gene screening for analyzing gene expression across goat tissue

Y Zhang, XD Zhang, X Liu, YS Li, JP Ding… - … journal of animal …, 2013 - ncbi.nlm.nih.gov
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the
changes in mRNA expression levels in cells and tissues. Selection of the proper reference …

Determination of novel reference genes for improving gene expression data normalization in selected canine reproductive tissues–a multistudy analysis

M Nowak, S Aslan, MP Kowalewski - BMC veterinary research, 2020 - Springer
Abstract Background Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative
measurement of gene expression. The proper choice of internal standards such as …

Evaluation of reference genes for quantitative real-time PCR in the brain, pituitary, and gonads of songbirds

WM Zinzow-Kramer, BM Horton, DL Maney - Hormones and behavior, 2014 - Elsevier
Quantitative real-time PCR (qPCR) is becoming a popular tool for the quantification of gene
expression in the brain and endocrine tissues of songbirds. Accurate analysis of qPCR data …

Validation of suitable reference genes for gene expression studies on yak testis development

X Zhou, X Wu, M Chu, C Liang, X Ding, J Pei, L Xiong… - Animals, 2020 - mdpi.com
Simple Summary Yak (Bos grunniens) provides life materials for herdsmen in high-plateau
areas. Improving their low fertility is necessary to meet the demands of the development of …

Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress

A Mahanty, GK Purohit, S Mohanty, NR Nayak… - BMC genomics, 2017 - Springer
Background High ambient temperature is known to affect fish gonadal development and
physiology in a variety of ways depending on the severity and duration of exposure; …

[HTML][HTML] PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows

HD Kim, CH Jo, YH Choe, HJ Lee, M Jang… - Animal …, 2022 - ncbi.nlm.nih.gov
Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the
most accurate and reliable technique for analysis of gene expression. Endogenous …