Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor

H Zhang, Z Liang, M Zhao, Y Ma, Z Luo… - … in Bioengineering and …, 2022 - frontiersin.org
Ectoine, an osmotic pressure-compensated solute, is used in the food, agriculture, medicine,
and cosmetics industries due to its ability to protect macromolecules. In this study, an ectoine …

Metabolic Pathway Construction and Optimization of Escherichia coli for High-Level Ectoine Production

J Chen, P Liu, X Chu, J Chen, H Zhang, DC Rowley… - Current …, 2020 - Springer
Ectoine is widely produced by various bacteria as a natural cell protectant against
environment stress, eg, osmotic and temperature stress. Its protective properties therefore …

Metabolic engineering combined with enzyme engineering for overproduction of ectoine in Escherichia coli

L Li, N Li, X Wang, S Gao, J Zhang, J Zhou, Z Wu… - Bioresource …, 2023 - Elsevier
Ectoine, a natural protective agent, is naturally synthesized at low titers by some extreme
environment microorganisms that are usually difficult to culture. There is a need for an …

Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli

Y Ning, X Wu, C Zhang, Q Xu, N Chen, X Xie - Metabolic engineering, 2016 - Elsevier
Ectoine is a protective agent and stabilizer whose synthesis pathway exclusively exists in
select moderate halophiles. A novel established process called “bacterial milking” efficiently …

Enhancing ectoine production by recombinant Escherichia coli through step-wise fermentation optimization strategy based on kinetic analysis

Y Dong, H Zhang, XY Wang, JJ Ma, P Lei, H Xu… - Bioprocess and …, 2021 - Springer
In this study, the recombinant ectoine-producing Escherichia coli ET01 was constructed by
introducing the ectABC operon from Halomonas venusta ZH. To further improve ectoine …

Highly efficient production of ectoine via an optimized combination of precursor metabolic modules in Escherichia coli BL21

S Xu, B Zhang, W Chen, K Ye, J Shen, P Liu, J Wu… - Bioresource …, 2023 - Elsevier
Ectoine is an osmotic pressure protectant observed in various microorganisms and is widely
used in cosmetics and pharmaceuticals. The market value of ectoine has increased …

Strategies for the biological production of ectoine by using different chassis strains

Y Feng, M Qiu, L Shao, Y Jiang, W Zhang… - Biotechnology …, 2023 - Elsevier
As an amino acid derivative and a typical compatible solute, ectoine can assist
microorganisms in resisting high osmotic pressure. Own to its long-term moisturizing effects …

Metabolic engineering of Escherichia coli for efficient ectoine production

S Zhang, Y Fang, L Zhu, H Li, Z Wang, Y Li… - Systems Microbiology …, 2021 - Springer
Ectoine is a high-value stabilizer and protective agent with various applications in enzyme
industry, cosmetics, and biomedicine. In this study, rational engineering strategies have …

[HTML][HTML] Engineering Escherichia coli for high-yield production of ectoine

D Wang, J Chen, Y Wang, G Du, Z Kang - Green Chemical Engineering, 2023 - Elsevier
Ectoine is a natural macromolecule protector and synthesized by some extremophiles. It
provides protections against radiation-mediated oxidative damages and is widely used as a …

Metabolic engineering of Escherichia coli for efficient osmotic stress‐free production of compatible solute hydroxyectoine

Q Ma, L Xia, H Wu, M Zhuo, M Yang… - Biotechnology and …, 2022 - Wiley Online Library
Compatible solutes are key for the ability of halophilic bacteria to resist high osmotic stress.
They have received wide attention from researchers for their excellent osmotic protection …