The transformative potential of gene editing technologies hinges on the development of safe
and effective delivery methods. In this study, we developed a temperature-sensitive and …

The CRISPR/Cas9 system is a versatile tool for functional genomics and forward genetic
screens in mammalian cells. However, it has been challenging to deliver the CRISPR …

The advent of RNA-guided endonuclease (RGEN)-mediated gene editing, specifically via
CRISPR/Cas9, has spurred intensive efforts to improve the efficiency of both RGEN delivery …

Strategies to modulate cellular DNA repair pathways hold immense potential to enhance the
efficiency of CRISPR-Cas9 genome editing platform. In the absence of a repair template …

The CRISPR/Cas9 technology has been rapidly developed as a gene‐editing tool by
manipulating nucleic acid sequences, which permits a precise gene knock‐out, knock‐in …

CRISPR-Cas9-mediated gene editing has vast applications in basic and clinical research
and is a promising tool for several disorders. Our lab previously developed a non-integrating …

The CRISPR/Cas9 system provides an easy way to edit specific site/s in the genome and
thus offers tremendous opportunity for human gene therapy for a wide range of diseases …

CRISPR-Cas12a nucleases have expanded the toolbox for targeted genome engineering in
a broad range of organisms. Here, using a high-throughput engineering approach, we …

T lymphocytes represent a promising target for genome editing. They are primarily modified
to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome …

The CRISPR/Cas9 systems have revolutionized the field of genome editing by providing
unprecedented control over gene sequences and gene expression in many species …