Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

Regulation of biological processes typically incorporates mechanisms that initiate and
terminate the process and, where understood, these mechanisms often involve feedback …

Physiological homeostasis becomes compromised during ageing, as a result of impairment
of cellular processes, including transcription and RNA splicing,,–. However, the molecular …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

Recent advances in sequencing techniques that measure nascent transcripts and that
reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …

Understanding how splicing events are coordinated across numerous introns in metazoan
RNA transcripts requires quantitative analyses of transient RNA processing events in living …

Production of mRNA depends critically on the rate of RNA polymerase II (Pol II) elongation.
To dissect Pol II dynamics in mouse ES cells, we inhibited Pol II transcription at either …

Alternative precursor messenger RNA (pre-mRNA) splicing plays a pivotal role in the flow of
genetic information from DNA to proteins by expanding the coding capacity of genomes …