Chemical tools have proven indispensable for studies in glycobiology. Synthetic
oligosaccharides and glycoconjugates provide materials for correlating structure with …

▪ Abstract In just a few short years, the chemical ligation of unprotected peptide segments in
aqueous solution has established itself as the most practical method for the total synthesis of …

The introduction of chemically unique groups into proteins by means of non-natural amino
acids has numerous applications in protein engineering and functional studies. One method …

The dissection and subsequent reassembly of a protein from peptidic fragments provides an
avenue for controlling its tertiary structure and hence its function. Here, we describe a …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

The technique of native chemical ligation enables the total chemical synthesis of proteins.
This method is limited, however, by an absolute requirement for a cysteine residue at the …

The ubiquitin (Ub)-proteasome system includes a large family of deubiquitinating enzymes
(DUBs). Many members are assigned to this enzyme class by sequence similarity but …

Although the reaction between an azide and a phosphane to form an aza‐ylide was
discovered by Hermann Staudinger more than 80 years ago and has found widespread …

The construction of complex protein folds relies on the precise conversion of a linear
polypeptide chain into a compact 3-dimensional structure. The interplay of forces that link …

We previously reviewed the use of 19 F NMR in the broad field of chemical biology [Cobb,
SL; Murphy, CD J. Fluorine Chem. 2009, 130, 132–140] and present here a summary of the …