CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

The continuing evolution of the SARS-CoV-2 virus has led to the emergence of many
variants, including variants of concern (VOCs). CRISPR-Cas systems have been used to …

The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …

Mutations in mitochondrial DNA (mtDNA) play critical roles in many human diseases. In vivo
visualization of cells bearing mtDNA mutations is important for resolving the complexity of …

Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type VA CRISPR-Cas anti-
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …

Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by
activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound …

Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to
counter-select against unedited cells. However, editing normally requires efficient …

CRISPR/Cas (clustered regularly interspaced short palindromic repeats linked to Cas
nuclease) technology has revolutionized many aspects of genetic engineering research …

CRISPR-based diagnostics (CRISPR-dx), including the Cas12-based DETECTR and Cas13-
based SHERLOCK Class 2 CRISPRs, have been used to detect the presence of DNA or …

Genome engineering nucleases must access chromatinized DNA. Here, we investigate how
AsCas12a cleaves DNA within human nucleosomes and phase-condensed nucleosome …