Several approaches for miRNA expression analysis have been developed in recent years.
In this article, we provide an updated and comprehensive review of available qPCR-based …

Arabidopsis pumila, an annual ephemeral plant, plays important roles in preventing wind
and sand erosion, water and soil conservation, and microhabitat improvement in the North of …

Cotton (Gossypium spp. L., Malvaceae) is the world's largest source of natural fibers. Virus
outbreaks are fast and economically devasting regarding cotton. Identifying new viruses is …

Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to
quantify gene expression. To achieve a reliable result, appropriate reference genes are …

The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind
prevention, and desert ecosystem recovery. An absence of reference genes greatly limits …

Background Cotton is one of the most important commercial crops as the source of natural
fiber, oil and fodder. To protect it from harmful pest populations number of newer transgenic …

Umbilical cord mesenchymal stem cells (UC-MSCs) exert strong immunomodulatory effects
and can repair organs. However, their roles in radiation injury remain unclear. We show that …

RNA sequencing (RNA-Seq)-based gene expression analysis is applicable to a wide range
of biological purposes in various species. Reverse transcription quantitative PCR (RT …

Main conclusion MicroRNAs miR390-5p, miR7694-3p miR1868 and miR1849 were found to
be suitable miRNA reference genes for rice, under either infection with the root-knot …

MicroRNAs (miRNAs) are short noncoding RNA molecules that regulate gene expression at
the posttranscriptional level. Reverse transcription-quantitative PCR (RT-qPCR) is one of the …