Are programmable freezers still needed in the embryo laboratory? Review on vitrification

G Vajta, ZP Nagy - Reproductive biomedicine online, 2006 - Elsevier
The predictable answer to the provocative question of whether programmable freezers are
still needed in the embryo laboratory is an even more provocative 'no'. However, such a …

Advances in Swine In Vitro Embryo Production Technologies

MA Gil, C Cuello, I Parrilla, JM Vazquez… - … in domestic animals, 2010 - Wiley Online Library
Contents Recent advances in new technologies to produce cloned and genetically modified
pigs involve manipulating oocytes and/or embryos in vitro. Although a great deal of progress …

Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics

L Mara, S Casu, A Carta, M Dattena - Animal Reproduction Science, 2013 - Elsevier
In the last few decades, farm animal genetic diversity has rapidly declined, mainly due to
changing market demands and intensification of agriculture. But, since the removal of single …

Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification

MK Gupta, SJ Uhm, HT Lee - Theriogenology, 2007 - Elsevier
Cryopreservation of normal, lipid-containing porcine oocytes has had limited practical
success. This study used solid surface vitrification (SSV) of immature germinal vesicle (GV) …

Achievements and future perspectives of embryo transfer technology in pigs

EA Martinez, CA Martinez, JM Cambra… - … in domestic animals, 2019 - Wiley Online Library
Commercial embryo transfer (ET) has unprecedented productive and economic implications
for the pig sector. However, pig ET has been considered utopian for decades mainly …

[HTML][HTML] Nonsurgical deep uterine transfer of vitrified, in vivo-derived, porcine embryos is as effective as the default surgical approach

EA Martinez, CA Martinez, A Nohalez… - Scientific Reports, 2015 - nature.com
Surgical procedures are prevalent in porcine embryo transfer (ET) programs, where the use
of vitrified embryos is quasi non-existent. This study compared the effectiveness of surgical …

Recent advances toward the practical application of embryo transfer in pigs

EA Martinez, C Cuello, I Parrilla, CA Martinez… - Theriogenology, 2016 - Elsevier
Porcine embryo transfer (ET) technology has been in demand for decades because of its
potential to provide considerable improvements in pig production with important sanitary …

[HTML][HTML] Effects of vitrification on the blastocyst gene expression profile in a porcine model

C Cuello, CA Martinez, JM Cambra, I Parrilla… - International Journal of …, 2021 - mdpi.com
This study was designed to investigate the impact of vitrification on the transcriptome profile
of blastocysts using a porcine (Sus scrofa) model and a microarray approach. Blastocysts …

Cryopreservation of porcine embryos: Recent updates and progress

D Xingzhu, Z Qingrui, C Keren, L Yuxi… - Biopreservation and …, 2021 - liebertpub.com
Cryopreservation of embryos is important for long-distance embryo transfer and
conservation of genetic resources. Porcine research is important for animal husbandry and …

[HTML][HTML] The open cryotop system is effective for the simultaneous vitrification of a large number of porcine embryos at different developmental stages

A Gonzalez-Plaza, JM Cambra, I Parrilla… - Frontiers in Veterinary …, 2022 - frontiersin.org
The Superfine Open Pulled Straw (SOPS) system is the most commonly used method for
vitrification of pig embryos. However, this system only allows the vitrification of four to seven …