Directed evolution methods for enzyme engineering

SR Nirantar - Molecules, 2021 - mdpi.com
Enzymes underpin the processes required for most biotransformations. However, natural
enzymes are often not optimal for biotechnological uses and must be engineered for …

Molecular engineering of antibodies for therapeutic and diagnostic purposes

F Ducancel, BH Muller - MAbs, 2012 - Taylor & Francis
During the past ten years, monoclonal antibodies (mAbs) have taken center stage in the field
of targeted therapy and diagnosis. This increased interest in mAbs is due to their binding …

Revolutionizing enzyme engineering through artificial intelligence and machine learning

N Singh, S Malik, A Gupta… - Emerging Topics in Life …, 2021 - portlandpress.com
The combinatorial space of an enzyme sequence has astronomical possibilities and
exploring it with contemporary experimental techniques is arduous and often ineffective …

A systems-level approach for metabolic engineering of yeast cell factories

IK Kim, A Roldão, V Siewers, J Nielsen - FEMS yeast research, 2012 - academic.oup.com
The generation of novel yeast cell factories for production of high-value industrial
biotechnological products relies on three metabolic engineering principles: design …

Protein engineering and its applications in food industry

S Kapoor, A Rafiq, S Sharma - Critical reviews in food science and …, 2017 - Taylor & Francis
Protein engineering is a young discipline that has been branched out from the field of
genetic engineering. Protein engineering is based on the available knowledge about the …

Recent advances in protein engineering and biotechnological applications of glutathione transferases

F Perperopoulou, F Pouliou… - Critical reviews in …, 2018 - Taylor & Francis
Abstract Glutathione transferases (GSTs, EC 2.5. 1.18) are a widespread family of enzymes
that play a central role in the detoxification, metabolism, and transport or sequestration of …

Genetically modified food enzymes: a review

Y Zhang, T Geary, BK Simpson - Current Opinion in Food Science, 2019 - Elsevier
Highlights•Food enzyme modification is developed by advanced genetic techniques.•
Genetically modified food enzymes can exhibit improved catalytic efficiency, stability …

Engineering of biocatalysts: from evolution to creation

MB Quin, C Schmidt-Dannert - ACS catalysis, 2011 - ACS Publications
Enzymes are increasingly being used in an industrial setting as a cheap and
environmentally friendly alternative to chemical catalysts. To produce the ideal biocatalyst …

Evolution of enzyme kinetic mechanisms

NN Ulusu - Journal of molecular evolution, 2015 - Springer
This review paper discusses the reciprocal kinetic behaviours of enzymes and the evolution
of structure–function dichotomy. Kinetic mechanisms have evolved in response to alterations …

Cognizance of molecular methods for the generation of mutagenic phage display antibody libraries for affinity maturation

CC Lim, YS Choong, TS Lim - International Journal of Molecular Sciences, 2019 - mdpi.com
Antibodies leverage on their unique architecture to bind with an array of antigens. The
strength of interaction has a direct relation to the affinity of the antibodies towards the …