Stereo-and regioselectivity in the P450-catalyzed oxidative tandem difunctionalization of 1-methylcyclohexene
GD Roiban, R Agudo, MT Reetz - Tetrahedron, 2013 - Elsevier
The selective partial oxidation of small non-functionalized molecules using biocatalysis
based on P450 monooxygenases is known to be difficult due to the expected poor regio-and …
based on P450 monooxygenases is known to be difficult due to the expected poor regio-and …
Behind the Scenes of PluriZyme Designs
Protein engineering is the design and modification of protein structures to optimize their
functions or create novel functionalities for applications in biotechnology, medicine or …
functions or create novel functionalities for applications in biotechnology, medicine or …
PCR mutagenesis and gap repair in yeast
M Weir, JB Keeney - Yeast Genetics: Methods and Protocols, 2014 - Springer
Random mutagenesis of a gene and subsequent screening for phenotypic properties is a
basic genetic procedure for studying gene function. When working with yeast, cloning …
basic genetic procedure for studying gene function. When working with yeast, cloning …
Methods and mechanisms for genetic manipulation of microorganisms
The omnipresence of microorganisms makes it important for us to discern the genetics and
mechanisms of them. Genetic manipulation acts as a tool for exploring gene functions …
mechanisms of them. Genetic manipulation acts as a tool for exploring gene functions …
High throughput mutagenesis for identification of residues regulating human prostacyclin (hIP) receptor expression and function
A Bill, EM Rosethorne, TC Kent, L Fawcett, L Burchell… - PLoS …, 2014 - journals.plos.org
The human prostacyclin receptor (hIP receptor) is a seven-transmembrane G protein-
coupled receptor (GPCR) that plays a critical role in vascular smooth muscle relaxation and …
coupled receptor (GPCR) that plays a critical role in vascular smooth muscle relaxation and …
Synthesis of fully protected trinucleotide building blocks on a disulphide-linked soluble support
R Suchsland, B Appel, P Virta, S Müller - Rsc Advances, 2021 - pubs.rsc.org
In recent years, preparation of fully protected trinucleotide phosphoramidites as synthons for
the codon-based synthesis of gene libraries as well as for the assembly of oligonucleotides …
the codon-based synthesis of gene libraries as well as for the assembly of oligonucleotides …
Enhancement of thermostability of Bacillus subtilis endoglucanase by error-prone PCR and DNA shuffling
MJ Yang, HW Lee, H Kim - Applied biological chemistry, 2017 - Springer
The Bacillus subtilis endo-β-1, 4-glucanase gene (beg), which encodes the enzyme BEG
that comprises 499 amino acid residues, was mutated by error-prone polymerase chain …
that comprises 499 amino acid residues, was mutated by error-prone polymerase chain …
[PDF][PDF] Advances in engineering of proteins for thermal stability
S Talluri - Int J Adv Biotechnol Res, 2011 - academia.edu
Proteins with increased thermal stability are desirable for many industrial, bioanalytical and
pharmaceutical applications. The thermodynamic thermal stability of a protein depends …
pharmaceutical applications. The thermodynamic thermal stability of a protein depends …
Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
P Eric, E Willert, J Kim, J Higgins - 2015 - Google Patents
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[C@@ H](O)[C@ H](O)[C@ H](O)[C@ H](O [C@@ H] 2 [C@@ H](O)[C@ H](O)[C@@ H](CO) …
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An efficient approach for site-directed mutagenesis using central overlapping primers
H Wang, N Zhou, F Ding, Z Li, R Chen, A Han… - Analytical biochemistry, 2011 - Elsevier
QuikChange is a popular method for site-directed mutagenesis in structural and functional
studies of proteins and nucleic acids. However, the standard protocol is often inefficient in …
studies of proteins and nucleic acids. However, the standard protocol is often inefficient in …