[HTML][HTML] Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method
MJ de la Cruz, ET Eng - Structure, 2023 - cell.com
Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …
[HTML][HTML] Cryo-focused ion beam for in situ structural biology: State of the art, challenges, and perspectives
AJ Noble, A de Marco - Current Opinion in Structural Biology, 2024 - Elsevier
Cryogenic-focused ion beam (cryo-FIB) instruments became essential for high-resolution
imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples …
imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples …
High-confidence 3D template matching for cryo-electron tomography
Cryo-electron tomography (CryoET) images the cellular environment in situ, without labels,
and with fully preserved context. However, the complex cellular environment and the low …
and with fully preserved context. However, the complex cellular environment and the low …
Doses for X‐ray and electron diffraction: New features in RADDOSE‐3D including intensity decay models
JL Dickerson, PTN McCubbin… - Protein …, 2024 - Wiley Online Library
New features in the dose estimation program RADDOSE‐3D are summarised. They include
the facility to enter a diffraction intensity decay model which modifies the “Diffraction …
the facility to enter a diffraction intensity decay model which modifies the “Diffraction …
Gentle ions for cryo-FIB milling
X Chu, M Kudryashev - Structure, 2023 - cell.com
Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness.
Focused ion beams can mill windows into cells and tissues for imaging, but they damage …
Focused ion beams can mill windows into cells and tissues for imaging, but they damage …
Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology
Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology,
enabling the study of macromolecules in their native cellular environments. However …
enabling the study of macromolecules in their native cellular environments. However …