Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …

Cryogenic-focused ion beam (cryo-FIB) instruments became essential for high-resolution
imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples …

Cryo-electron tomography (CryoET) images the cellular environment in situ, without labels,
and with fully preserved context. However, the complex cellular environment and the low …

New features in the dose estimation program RADDOSE‐3D are summarised. They include
the facility to enter a diffraction intensity decay model which modifies the “Diffraction …

Cryo-EM imaging of vitreous samples is limited to a few hundred nanometers in thickness.
Focused ion beams can mill windows into cells and tissues for imaging, but they damage …

Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology,
enabling the study of macromolecules in their native cellular environments. However …